We narrowed to 156 results for: ado
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TypeBlog Post...happens (this time, Poisson statistics), and the readout gives an absolute value of DNA concentration. By.... PMID: 21554069. Haridan, U. S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., Shueb, R. H., Zaid, ...
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Light Sheet Fluorescence Microscopy
TypeBlog Post...concentration of fluorophores) cast shadows along the illumination path. The shadows appear as stripes in the acquired... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post....1038/s41597-021-00981-0 Wright, J. F., Le, T., Prado, J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., ...L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A.... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...number of bands present on the strip. This type of readout allows for nucleic acid detection almost anywhere...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...from the yeast S. cerevisiae (Gronostajski and Sadowski 1985). The FLP system works in a similar fashion...PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP Recombinase of the Saccharomyces... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...escape a threat. Locomotion can thus be used as a readout for associated emotional phenomena like stress ...2018). Locomotion can also be used as behavioral readout to study certain forms of drug addiction. When ... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...approach can be incorporated into a lateral-flow readout slip that allows rapid detection. Highlights: ...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ... -
Luciferase Plasmid Collection
TypeCollection...Plasmids from 2016 blog post. LuTHy plasmids (Double‐Readout Lu minescence‐based T wo‐ Hy brid) : Combines in... -
Antibodies 101: Validation
TypeBlog Post...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018; Uhlen, et...Nature 518:27–9. https://doi.org/10.1038/518027a Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... the intended target site. With the widespread adoption of CRISPR/Cas9 technology and rapid improvements... field (DAPI). Using the ICC assay as a direct readout of tag insertion efficiency, we have recently demonstrated... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...targeted cells. Depending on the cells used and the readout, this could be a major limitation. An alternative...custom CRISPR screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by ... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...have the proper controls or a proper behavioral readout that allows you to interpret the results of your... high quality stereotaxic frame with a digital readout. The advantage is you can “zero” your coordinates... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...inducible gene expression Brilliant technologies adopt basic biological concepts and employ them in creative...copies of targeting sequences. Scientists have adopted and employed this concept in both imaging and inducible...observed at a later time via DNA sequencing or other readout. Cellular memory is accomplished by the use of ... -
Using AAV for Neuronal Tracing
TypeBlog Post...., Greenbaum, A., Ravi, N., Wu, W.-L., Sánchez-Guardado, L., Lois, C., Mazmanian, S.K., Deverman, B.E....PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R. J., and Tuszynski... -
Technical Design of a Western Blot
TypeBlog Post...easy to source, making it easy to switch from one readout method to another or change primary antibodies ...this truly does provide a relative, not absolute, readout. Normalization Overall protein expression varies... -
Antibody Guide
TypeCollection...Sample preparation, signaling excitation, and assay readout vary widely depending on individual assay conditions... -
CRISPR Guide Update
TypeBlog Post...incredible new CRISPR technologies developed and widely adopted in the past few years, as well as refreshing the... -
Trends in CRISPR and SynBio Technologies
TypeBlog Post...dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly accelerated... -
#Deckthelab Contest Winners 2017
TypeBlog Post...never forget! The Mackin Lab from University of Colorado -Anschutz Medical Campus (@CSD_CUAMC) caught a... -
CRISPR 101 eBook...en español
TypeBlog Post...Daniel Mejías Sánchez, ¡y no podríamos estar más emocionados! Después de recibir la traducción, Addgene contrató...