We narrowed to 50 results for: astl

1. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   ... A to distinguish single cells from duplicates. Lastly, you learned which gates should always be included...

2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   Blog Post

   ...ideally be around 5 to 20 bp away from the cut site. Lastly, if a heterozygous event is desired but only guide...

3. Site Directed Mutagenesis by PCR

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   Blog Post

   ...ablation) of a restriction site through mutagenesis vastly facilitates the subsequent process of screening...

4. Treating Muscular Dystrophy with CRISPR Gene Editing

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   Blog Post

   ...-type level. Muscle histology is improved, with vastly reduced inflammation and necrosis compared to unedited...

5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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   Blog Post

   ...Materials and Methods section of our paper (1). Lastly, it is possible to build gene regulatory RSMs (...

6. Plan Your Experiment

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   Guide

   ...generating modified mouse lines. Protein delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP...pool of cells to find one with your desired edit. Lastly, you will need to know if your edit just affects...

7. Important Considerations When Using AAVs

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   Blog Post

   ... 24086725. PubMed Central PMCID: PMC3785459. 4. Castle, Michael J., et al. "Adeno-associated virus serotypes...

8. Typing CRISPR Systems

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   Blog Post

   ...fragments of foreign DNA into the CRISPR array. Lastly, type IV systems are most often found on plasmids...

9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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   Blog Post

   ...cells. A point to keep in mind- although PA-FPs are vastly improved over the conventional FPs for super-resolution...

10. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...entirely. If you are trying to transfer proteins of vastly different sizes, you may need to optimize quite...