We narrowed to 70 results for: calcium

1. Hot Plasmids and Viral Preps - March 2021

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   ...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

2. Overcoming the Challenges of Lentiviral Production

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   ...the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of ...

3. Fluorescent Proteins 101: Introduction to FRET

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   ..., 1997), was designed to measure intracellular calcium and published in 1997. Since that time, numerous...

4. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ... Lysine (D123K), lowers biosensor affinity for calcium.”  Primary Vector Type The vector type should...

5. Hot Plasmids - October 2020

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   ...AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ...

6. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   ...transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed...

7. Which Fluorescent Protein Should I Use?

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   ... commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin...

8. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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   ...18845710. Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming...

9. Plasmids 101: The Promoter Region – Let's Go!

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   ...Used for neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA...

10. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ...and determine the topology of the mitochondrial calcium uniporter (Martell et al., 2012). It is important...

11. TALENs for Endogenous Zebrafish Genes

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    ...TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA NADPH oxidase, EF-hand calcium binding domain 5 TAL3496 & TAL3497 TGCCGCCAACTGGCTGAAacctccagatctggagCAGAACAAGCGCAAAA...

12. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...Hartmann, and Peter Hegemann. "Rhodopsin-regulated calcium currents in Chlamydomonas." Nature 351.6326 (1991...

13. Special Delivery: Fluorophore Targeting for FRET Studies

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    ...Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring...

14. How to Deposit Your Plasmids with Addgene

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    ... Lysine (D123K), lowers biosensor affinity for calcium". Please describe any tags or fusions (e.g. His...

15. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    ..., et al. "Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging." Nature...

16. Luciferase Plasmid Collection

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    ..., and Nanoluc®. CalFlux VTN : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore...

17. Deisseroth INTRSECT Collection

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    ...excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases...

18. Chemogenetics Guide

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    Guide

    ... phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based...

19. Fluorescence Titering Assay

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    Protocol

    ...mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...

20. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    ...light-inducible heterodimerizing cryptochrome 2 (CRY2) and calcium and integrin-binding protein 1 (CIB1) proteins....