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We narrowed to 715 results for: cas

Showing: 41 - 60 of 715 results

  1. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post
    ...Separate Cas proteins then express and process the CRISPR loci to generate RNA, which guide a Cas nuclease.... Many CRISPR/Cas moieties carry out these functions using slightly different Cas proteins and PAM ...CRISPR/Cas defense by generating a single point mutation in the PAM sequence, preventing the Cas nucleases... information on CRISPR/Cas, check out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted...clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular genome...look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive defense strategy against...

  2. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post
    ... Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for...missing: a way to shut off Cas’s activity. The concern is that the longer Cas remains active in a cell,...protein inhibitors of CRISPR-Cas systems that help phages evade the CRISPR-Cas immune system of bacteria.... II-A CRISPR-Cas systems. A full list of Acr proteins organized by the type of CRISPR-Cas system they ...that interact with CRISPR-Cas systems such as Cascade-Cas3, Cas9, Cas12, and Cas13 (Marino et al., 2020)....AcrIIA4 blocks Cas9’s interaction with the PAM site (Dong et al., 2017), and AcrIIC3 causes Cas proteins to...interactions with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al...

  3. CRISPR 101: Off-Target Effects

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    ...mutations arise from base/prime editing or Cas cleavage. In the case of Cas cleavage, off-target mutations can...confer specificity of the nuclease; however, many Cas and Cas-like nucleases are promiscuous. In other words...consider your Cas enzyme. Even if there are similar sequences present in the genome, certain Cas enzymes are...approaches have been developed. Cas ChIP-Seq, where only DNA sites bound by Cas are sequenced, has been tested...appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding...CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) CRISPR 101... of Maya Kostman. The most commonly used Cas, spCas9, has gone through multiple rounds of optimization...

  4. Typing CRISPR Systems

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    Blog Post
    ...type VI, each relying on a unique Cas effector — Cas9, Cas12, or Cas13. If you’d like to explore Class ...while Class 2 use single protein Cas effectors, like Cas9. The Cascade complex doesn’t usually carry out...attributed to the multi-Cas effector, Cascade. Developing tools using a Cascade complex is tricky, as you...the blog CRISPR 101: Cas9 vs. The Other Cas(s) CRISPR 101: RNA Editing with Cas13 Build your CRISPR vocabulary...Class 1 CRISPR systems use multi-Cas effectors, referred to as Cascade (CRISPR-associated complex for antiviral... subtypes use Cas10 as the primary effector complex but are defined by the accessory Cas proteins in the...which are among the smallest Cas enzymes (400 to 700 amino acids), use a Cas14 enzyme, and uniquely target...

  5. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

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    Blog Post
    ...gene cas14. cas14 encodes for a small Cas protein (40-70 kDa), which is half the size of other Cas proteins...characteristic of CRISPR-Cas enzymes. In contrast to other Cas enzymes, Cas14 has not been found in bacterial...integrase, cas1. They discovered a family of CRISPR-Cas systems containing cas1, cas2, cas4, and a new...CRISPR-Cas systems. There are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All.... Identifying Cas14 variants In this quest to discover new and maybe more efficient Cas systems, Jennifer...of virtually any organisms on this planet, CRISPR-Cas systems were merely adaptive immune systems that ...try to determine whether simpler and maybe smaller Cas systems could exist in nature. To do this, they ...

  6. Build Your CRISPR Vocabulary

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    Blog Post
    ...Protein Cas: Different CRISPR systems involve different proteins, but many of them are called Cas, short...by the Cas9 protein from the appropriate species. Some Cas proteins, like LbCas12a or AsCas12a, do not...encodes a tracrRNA (in some systems including Cas9), Cas proteins, and a CRISPR array. The CRISPR array...spacer. In bacteria, these crRNAs are bound by Cas proteins (Cas9 shown here). The repeat sequence base pairs... famous of these proteins is Cas9 nuclease, but there are many other Cas proteins as well, both natural...organized. Target strand: The strand of foreign DNA that Cas proteins recognize using an RNA guide. Non-target... to be cut, which varies between CRISPR systems. Cas proteins won’t cut DNA that doesn’t have their specific...

  7. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ...-flexible Cas enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or ...placement significantly over SpCas9 and more so than Cas9s with an NG PAM.   Cas PAM sequence  Additional...Expanding Beyond Cas9 xCas9: Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale ...are some available.   Cas proteins that don’t utilize NGG   There are plenty of Cas9 variants which utilize...etc.). Additionally, alternative Cas enzymes exist, such as Cpf1/Cas12a, which recognizes the PAM sequences... of generating Cas enzymes with alternative PAM requirements (Zhao et. al, 2023). xCas9 has been shown...Walton et. al, 2020). These Cas proteins have everything we know and love about Cas9, but with broader PAM ...

  8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post
    ...catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case a deaminase...change before the Cas domain lets go of the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold... are only weakly compatible with many Cas domains beyond SpCas9. In order to expand the gene-editing applications...specifies the editing target site on the DNA, the Cas domain directs the modifying enzyme to the target...substitutions.  The need for speed: base editing before Cas lets go Figure 1: David Liu gives a talk at...to evolve ABEs with an increased compatibility to Cas homologs. Evolving better base editors with the help...deamination rate so that deamination can happen before Cas homologs let go. The Liu lab developed the Phage-...

  9. What's New in CRISPR - Winter 2018

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    Blog Post
    ...engineered variants of Cas nucleases. In doing so, they also created a modular toolkit for Cas-mediated genome...editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family of small...small DNA-targeting Cas proteins. Cas14 targets and cleaves ssDNA which they have adapted for detecting...genome editing in plants While there have been many Cas nucleases developed for plants, it has been difficult...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with ...compared to monomeric Cas9’s. Read the paper in Nature Communications Find the Cas9-Cas9 chimera plasmids...Science Find the Cas14 plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR ...

  11. Components of CRISPR/Cas9

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    ... targeting specificity). Cas9 endonuclease functions While native CRISPR/Cas systems have a variety of...sometimes sgRNA) In the native Type II CRISPR/Cas system, Cas9 is guided to its target sites with the aid...vary (for example, xCas9 is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased...sequence, Cas9 will neither bind nor cut the target. Cas9 homologs from different organisms or Cas9 mutants...pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant...3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant  3' NGAN or NGNG  xCas9 3' NG, GAA,...basic level, CRISPR/Cas9 genome editing systems use a non-specific endonuclease (Cas9 or closely related...

  12. Keeping Up With CRISPR/Cas9

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    ...Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. Ahmed A. Gomaa, et al. mBio. 2014 January...CRISPR/Cas9 system to precisely edit specific genes in monkeys. "Our study shows that the CRISPR/Cas9 system...for CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References, Protocols, and ...advances in the understanding and application of CRISPR/Cas9 technology are coming along at a fast and furious...Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing an antibiotic that selectively...important details in their understanding of how CRISPR/Cas9s actually work. Last month, the Lawrence Berkeley... Nogales presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography...

  13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...multiple Cas proteins to form the Cascade targeting complex is a hallmark of Type I CRISPR-Cas systems,... (such as CRISPR-Cas9). Unlike in regular Type I CRISPR-Cas systems, however, Cascade from VchINT does...to Type I-F, but instead employ a Cas12k protein instead of three Cas proteins (Fig. 1A, bottom), and require...transposases and the target programmability of CRISPR-Cas, these systems represent the next generation of tools... hereafter VchINT) and encodes a Type I-F CRISPR-Cas system. The four major components required for RNA-guided... of DNA targeting by a transposon-encoded CRISPR–Cas system. Nature 577:271–274 . https://doi.org/10.1038...TS, Sternberg SH (2019) Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration. Nature...

  14. 3D Printing Meets CRISPR Cas9

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    Blog Post
    ...shifts. This backbone model of Cas9 is based on Fuguo Jiang’s structure of Cas9 – primed for cleavage. This...researchers for the past fifteen years. On those occasions when we are fortunate to hand-deliver the model...contacted us in 2014 to ask if we could make a model of Cas9 based on the structure published by Martin Jinek...other aspects of the structure. While this first Cas9 model is certainly useful, it is also wrong in at... at least one serious way. We represented the Cas9 protein in a surface format made of rock-hard, inflexible... the amazing conformational flexibility that the Cas9 protein must have in order to scan along the double-stranded...model of a protein. We now have another model of Cas9 – a flexible alpha-carbon backbone model made of...

  15. A Case Study of Addgene Bioinformatics

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    Blog Post
    ... sequencing data.”  Recently, Seqera published a case study on how Addgene has used Nextflow to improve...our existing resources. You can read it, and other case studies, on the Seqera website. ...

  16. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...Cambridge, MA and Sequana Therapeutics in San Diego, CA. She earned a B.S in Biology from MIT.   Kenneth...at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie Chen is a co-founder of Addgene and received... In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and...Listen to the interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog... podcast...

  17. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post
    ...loci in living human cells by an optimized CRISPR/Cas system. Chen B, Gilbert LA, Cimini BA, Schnitzbauer... a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...states. dCas9 gets a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9... colorful system that uses fluorescently labeled Cas9 to label multiple genomic loci in live cells. While...hybridization (FISH) or fluorescently labeled TALEs, CRISPR/Cas9’s ease of use and ability to label live cells make...well-known being transcriptional activation/repression. dCas9 has also previously been adapted to fluorescently...required by each ortholog, a gRNA designed for one dCas9 should be specific to that ortholog and not cross-talk...

  18. CRISPR/Cas9 FAQs Answered!

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    Blog Post
    ...puromycin resistance cassette, such as PX459). After you co-transfect the CRISPR/Cas system with your homologous...article list, the Genome Engineering Using CRISPR/Cas Systems has expanded out of control. To further consolidate...that can be inserted into the genome using CRISPR/Cas for Homologous Recombination (HR)? How long should...2013). Multiplex genome engineering using CRISPR/CAS systems. Science, 339(6121), 819–823. https://doi.org....2013.143 Sander, J. D., & Joung, J. K. (2014). CRISPR-Cas systems for editing, regulating and targeting genomes...mice carrying mutations in multiple genes by CRISPR/CAS-Mediated Genome Engineering. Cell, 153(4), 910–918...carrying reporter and conditional alleles by CRISPR/CAS-Mediated Genome Engineering. Cell, 154(6), 1370–1379...

  19. CASTing Off for New Shores in Human Genome Editing

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    ...Mechanism of CAST systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K systems; Cascade shown...nuclease-deficient Cas12k (Gelsinger et al., 2024). In either type of CAST, the nuclease-deficient CRISPR-Cas system...CRISPR-Cas systems to help them spread through bacterial genomes (Peters et al., 2017). Type I CAST systems...payload sequence into genomic DNA downstream from the Cas target. Conveniently, the process doesn’t cause double-stranded...Sternberg, S. H. (2019). Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration. Nature...., & Koonin, E. V. (2017). Recruitment of CRISPR-Cas systems by Tn7-like transposons. Proceedings of the...called evoCAST — an evolved CRISPR-associated transposase (CAST). Back up a second. What are CASTs? CASTs...

  20. What's New in CRISPR - November 2021

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    Blog Post
    ...CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (CasMINI) engineered...Miniature CRISPR-Cas systems SEND plasmids Prime editing improvements Miniature CRISPR-Cas systems By Brook... yet active Cas system starting with the same Un1Cas12f1 (Cas14). They noted that Cas12f1 had an extra-long...  They did not modify the Cas12f1 enzyme. The result is a highly efficient Cas system with very compact...miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature Cas system that utilizes...the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI system was developed by engineering... enzyme with a fully active Cas9 enzyme. PE-Cas9 comprises an active Cas9 fused to the reverse transcriptase...
Showing: 41 - 60 of 715 results