We narrowed to 200 results for: ckb

...blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies on this plate...list of Bacterial Expression Systems Find Empty Backbones for Your Experiments    ...

...while expressing the gene. Briefly, the vector “backbone” in this system contains ccdB. A successful insertion...propagate and prep your ccdB-containing empty backbones but should never be used when selecting for recombinant...

...sequence and protein data, as well as available backbone sequences. All of these resources allow QC scientists...feature highlighted by the deposited scientist  The backbone elements based on the information provided and...

...that target the common features of viral vector backbones; once validated these universal primers can be... for functional assays depending on the vector backbone. When using qPCR based methods for viral titration...

...tagging the kinesins KIF5A and KIF5B as well as backbones for generating other SNAPf-, CLIPf-, and CLAP-...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload...

...the desired mutation and excision of the vector backbone. To do this, rhamnose is added which induces expression...point will be those that have excised the vector backbone. Vectors pTOX4, pTOX5, and pTOX6 express the ...

...plasmid backbone that was designed specifically for transient expression (find some useful backbones here...

...not leave much flexibility for discrepancies in backbone sequences. Most theoretical sequences submitted...deposits that contain many plasmids within the same backbone. The flexible auto-alignment, plus additional ...

...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

...protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

...track: Compatibility: Double-check your plasmid backbone and expression strain to make sure they are compatible...below or browse Addgene’s collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin...

...respective species. Addgene carries >30 “empty gRNA” backbones which contain a plant pol III promoter and gRNA...plants and many of the aforementioned empty gRNA backbones also contain Cas9, which enables expression of...

...interest (Figure 2). 1. The phosphoserine OTS The backbone of the phosphoserine OTS, Sep-OTS, contains the...using the PhosTag system (see below). This plasmid backbone can also be cloned with an ORF of interest for...

...chemically modify target bases without breaking the DNA backbone, making them efficient and precise tools for altering...which incorrect nucleobases are removed from the backbone, creating abasic sites for further repair.   ...

...thymine in this case) is only bound by the phosphate backbone. These PAM bases must be present for Cas9 to unwind... both lobes bind to the DNA and RNA phosphate backbones, allowing Cas9 to verify the structure of the ...

...Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope protein for producing lentiviral and...

...full list here. Most of these plasmids are empty backbones, some of them containing fluorescent markers or...

...cloning of your gene of interest into a plasmid backbone. Transfection grade DNA Figure 3: Need...

... genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus...

...reaction. STAgR is highly customizable as vector backbones, and gRNA structure, numbers, and promoters can...