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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 200 results for: ckb

Showing: 41 - 60 of 200 results

  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies on this plate...list of Bacterial Expression Systems Find Empty Backbones for Your Experiments    ...

  2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...while expressing the gene. Briefly, the vector “backbone” in this system contains ccdB. A successful insertion...propagate and prep your ccdB-containing empty backbones but should never be used when selecting for recombinant...

  3. A Look at Addgene's QC Process

    Type
    Blog Post
    ...sequence and protein data, as well as available backbone sequences. All of these resources allow QC scientists...feature highlighted by the deposited scientist  The backbone elements based on the information provided and...

  4. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...that target the common features of viral vector backbones; once validated these universal primers can be... for functional assays depending on the vector backbone. When using qPCR based methods for viral titration...

  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...tagging the kinesins KIF5A and KIF5B as well as backbones for generating other SNAPf-, CLIPf-, and CLAP-...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload...

  8. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    ...protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

  10. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...not leave much flexibility for discrepancies in backbone sequences. Most theoretical sequences submitted...deposits that contain many plasmids within the same backbone. The flexible auto-alignment, plus additional ...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...track: Compatibility: Double-check your plasmid backbone and expression strain to make sure they are compatible...below or browse Addgene’s collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin...

  12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...respective species. Addgene carries >30 “empty gRNA” backbones which contain a plant pol III promoter and gRNA...plants and many of the aforementioned empty gRNA backbones also contain Cas9, which enables expression of...

  13. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...interest (Figure 2). 1. The phosphoserine OTS The backbone of the phosphoserine OTS, Sep-OTS, contains the...using the PhosTag system (see below). This plasmid backbone can also be cloned with an ORF of interest for...

  14. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...thymine in this case) is only bound by the phosphate backbone. These PAM bases must be present for Cas9 to unwind... both lobes bind to the DNA and RNA phosphate backbones, allowing Cas9 to verify the structure of the ...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...chemically modify target bases without breaking the DNA backbone, making them efficient and precise tools for altering...which incorrect nucleobases are removed from the backbone, creating abasic sites for further repair.   ...
Showing: 41 - 60 of 200 results