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Addgene

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In Category

We narrowed to 114 results for: dcas9

Showing: 41 - 60 of 114 results

  1. Editor's Choice, August 2016

    Type
    Blog Post
    ...uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at...

  2. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...coupling a cytidine deaminase with the inactive dCas9 (Komor et al., 2016). These fusions convert cytosine...an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change.... To do so, they used a Cas nickase, instead of dCas9. The resulting editor, BE3, nicks the unmodified...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... The popular CRISPR base editing technique uses dCas9/Cas9 nickase fused to a cytidine deaminase for targeted...Each individual strain includes a xylose-inducible dCas9 paired with a single, unique, and constitutively... applications, such as reversing the effects of dCas9 binding to a genomic locus, or limiting the amount...

  4. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene....position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Nucleic Acids...

  7. Fluorescence Titering Assay

    Type
    Protocol
    ...transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...diversification by targeting a site of interest with dCas9 fused to the Activation Induced Cytidine Deaminase...

  16. Lentivirus Production

    Type
    Protocol
    ...per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would...

  17. Colony Formation Titering Assay

    Type
    Protocol
    ...serial dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were treated with...
Showing: 41 - 60 of 114 results