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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 115 results for: dcas9

Showing: 41 - 60 of 115 results

  1. CRISPR Guide

    Type
    Guide
    ...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent...human cells. The simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single...imaging of proteins in living cells (Figure 9B) dCas9-VPR - dCas9 fused to several different activation domains...utilizes biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin... defense CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator with a gRNA to increase transcription...target gene CRISPRi CRISPR Interference; using a dCas9 or dCas9-repressor with a gRNA to repress/decrease transcription...one strand. Catalytically inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene....position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Nucleic Acids...

  4. Plan Your Experiment

    Type
    Guide
    ...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different ...edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with different base editing...be more effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene... the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators that need...N. J. (2023). Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent...

  5. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...coupling a cytidine deaminase with the inactive dCas9 (Komor et al., 2016). These fusions convert cytosine...an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change.... To do so, they used a Cas nickase, instead of dCas9. The resulting editor, BE3, nicks the unmodified...

  7. Fluorescence Titering Assay

    Type
    Protocol
    ...transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...diversification by targeting a site of interest with dCas9 fused to the Activation Induced Cytidine Deaminase...

  16. Lentivirus Production

    Type
    Protocol
    ...per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would...

  17. Colony Formation Titering Assay

    Type
    Protocol
    ...serial dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were treated with...
Showing: 41 - 60 of 115 results