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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 142 results for: endog

Showing: 41 - 60 of 142 results

  2. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ... the bait. Also, RaPID can’t be used to study endogenous RNAs at their physiological expression levels...levels; however, knock-in of BoxB sites into endogenous DNA sequences could allow one to study RNAs expressed...

  3. Design Tips for Prime Editing

    Type
    Blog Post
    ...pegRNAs (but not epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). ...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

  4. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...signaling can also modify other properties such as endogenous gene expression, which can lead to changes in...with the goal of creating receptors with less endogenous activation and reduced off target activation ...

  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...heterotrimers with endogenous Gβγ, enabling detection of activation triggered by endogenous GPCR. Example ...

  6. MXS Chaining

    Type
    Blog Post
    ...methods are not necessarily suitable for combining endogenous sequences. Table 2: High Throughput Cloning Strategies...modules themselves (so may not be suitable for endogenous coding sequences), so enzyme choice is critical...

  7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...conjugated to molecular species with high affinity for endogenous molecular targets such as paclitaxel. Unlike ...required as the small-molecule binding motif targets endogenous protein (Figure 1f). Labeling strategy pros...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...typically expressed at much higher levels than endogenous proteins. Data obtained from experiments using...tagged protein may not be translatable to the endogenous system and will need to be verified using alternative...

  9. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...abasic (empty) DNA site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various...efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient enough to hinder adenine...

  10. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...the target antigen. Some examples could be an endogenous sample of wild type cells, purified protein, ...be sure to include a sample that expresses the endogenous protein, since folding of the recombinant protein...

  11. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...which are cleaved out of the RNA transcript by endogenous processing enzymes (Figure 1). To ensure this...et al. (2024) Improving prime editing with an endogenous small RNA-binding protein. Nature. 2024 Apr 3...

  12. TALEN Engineering

    Type
    Collection
    ...-Fast and FLASH TALENs for Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the...

  14. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...to a histone tail to recruit and stimulate the endogenous methyltransferase. The strategy is generalizable... protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited methyltransferase DNMT3A to the...

  15. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...Tailored mutations are commonly introduced to endogeneous DNA through homology-directed repair (HDR) of...editing requires a template of high homology to the endogenous target, yet to facilitate the repair, the template...

  16. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins...-specific editing of histone modifications at endogenous enhancers. Nature Biotechnology 31:1133–1136 ...

  17. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...reaction. In fact, NLuc is bright enough to detect endogenous tagged genes generated through CRISPR/Cas9 knock-in...knock-in. Finally, NLuc is very inviting for endogenous protein tagging as it is only 19 kDa. An example...

  18. A Control for All Seasons

    Type
    Blog Post
    ...albumin. Secondary antibodies can also react with endogenous antibodies naturally present in the sample, for...experimental run (Burry 2011). Autofluorescence Endogenous fluorescence of the sample, or autofluorescence...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...that tests enhancer sequences outside of their endogenous chromatin environment and relies on the fact ...because it was difficult to fuse the AID tag to endogenous proteins. As described in their recent Cell Reports...genes of interest to generate mAID fusions to endogenous genes. Cell lines with the appropriate fusions...

  20. Worm Expression Resources

    Type
    Collection
    ...Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR ...Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even more elegant: Single ...
Showing: 41 - 60 of 142 results