Split Fluorescent Proteins for Studying Protein-Protein Interactions
Type
Blog Post
...General Design
Figure 1: Two proteins are fused to the FP(11) and FP(1-10) fragments. When Protein...and 11 to yield large (1-10) and small (11) fragments. In several cases an FP(1-10) fragment may be compatible...Description
Article
PI
Blue
EBFP2
EBFP2(1-10) and Capri(1-10) for use with GFP(11)
Multiplexed labeling...Commun. 2017
Bo Huang
mNeonGreen3
mNG3K(1-10) and mNG3A(1-10) for use with mNG2(11)
Improved yellow-...sfCherry2
sfCherry2(1-10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1-10)
Improved split...brings the pieces of the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence...even from a different base FP (for example, EBFP2(1-10) can be used with GFP(11)). Some of the articles...