We narrowed to 191 results for: hwa

...to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways Efflux Pumps...Synthetic Biology plasmids for metabolic enzymes and pathways. Plasmid...

...light to activate, inhibit, and control molecular pathways or cells. Chemogenetics Guide | Chemogenetics ...engineered receptors that affect cell signalling pathways. Luciferase Plasmids Use bioluminescent reporters...reporters for studying gene expression, signaling pathways, and more. Learn More and Explore Addgene Blog:...

...kynurenine pathway of tryptophan degradation. It turns out that activation of the PGC-1a1 pathway increases...protected. Effects of Activation of the PGC-1a1 Pathway Gene expression analysis of the animals’ skeletal...

...approaches. The first route involves identifying a gene/pathway to develop a drug for (aka a druggable target)....reveal individual genes or sometimes even genetic pathways that are worth investigating for drug development...’ proteins – having multiple hits or an entire pathway of possibilities greatly increases the odds of ...

...the Gβγ dimer, activating downstream signaling pathways.  This dissociation can be measured using a technique...system. Conclusions Although the importance of GPCR pathways is well known and, as a result, a target of many...collection can be used to study endogenous ligands, the pathways they activate, as well as the activation by drugs...

...need to learn much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage...finally grasped just how complicated DNA repair pathways are. I fully admit to being a DNA repair newbie...template strategies activate different HDR sub-pathways like synthesis-dependent strand annealing (SDSA...

...the preferred pathway for generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing... counterpart, enabling study of human-relevant pathways (Liu et al., 2017). Beyond human relevance, yeast...

...repaired by the non-homologous end-joining (NHEJ) pathway disrupt target gene sequences (thus inactivating...breaks repaired by homology directed repair (HDR) pathways can modify the sequence of a gene (thus altering...Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab Members who worked on ...

...potential toxicity and cross-reactivity with other pathways. Since these effectors are diffusible, they’re...input patterns may also be used to engineer new pathways with sophisticated gene expression controls. Most...inducible promoters that could crosstalk with other pathways. They also reduced system leakiness and increased...

... process. The process can range from a defined pathway or reaction to a complex event such as cancer or...acquired a favorable mutation. But if the cellular pathway of interest conveys no significant survival advantage...advantage, such as a non-essential mechanism or any pathway with cellular redundancies, a screen would be required...

...metabolic engineering, the expression of enzymes in a pathway will ideally be optimized to promote generation... the shuttling of the intermediates into other pathways. In cases where a specific ratio of products is...variability between the different components. When your pathway needs precise fine-tuning of gene expression levels...

...etc., microbially or in cell-free systems. These pathways typically involve multiple steps in which a precursor...isolated afterwards. To engineer these production pathways, MoClo has been used to assemble multigene constructs...testing any number in the Level 2 assembly. These pathways can require a lot of optimization - which is why...

...cell viability and increase protein production (Schwarz et. al., 2020). Harvest and purification At its....2013.00217. PMID: 23908655; PMCID: PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, ...Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau ...

...several proteins in the DNA oxidation and repair pathways work downstream of Tet1 to restore the DNA after... S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015) Stable oncogenic silencing...coupling of methylcytosine oxidation and DNA repair pathways. Nature Communications 10:. https://doi.org/10.1038...

...vectors - which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...Recombination or Alternative End Joining, two alternative pathways. See (Guo et al, 2023) for a review of other targeted...to their location (e.g. in a gene in a similar pathway, a growth factor, etc.) this control is usually...

...breaks into the homologous recombination repair pathway. Reagents required to perform pha-1 co-conversion...researchers to quickly and easily start genome editing (Schwartz et al., 2018). To see more CRISPR tools for C.... . https://doi.org/10.1534/genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing...

...advantage of repair pathways within the cell — in some cases, the same repair pathways that were considered...transversion editors rely on a multi-step cellular repair pathway that is known for sometimes causing double-strand...

...Return to top Membrane Trafficking The secretory pathway is responsible for delivering proteins to the extracellular... shipped. On the other hand, the endolysosomal pathway recylces internal membrane-bound structures and...markers for components of these membrane trafficking pathways and structures. ID Plasmid Structure Targeting...

...demonstrating that this system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin... M., Nguyen, T. H., Prathima, P., Fithian, W., Schwartz, M. A., Hacohen, N., Doench, J. G., LaFleur, M...

... will be able to better choose which genes and pathways to target in future small molecule screens. He...coworkers to test inhibitors of a variety of signaling pathways for their effects on the disease. This work will...