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We narrowed to 188 results for: hwa

Showing: 41 - 60 of 188 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...functions, from endosomal trafficking to signaling pathways to the cell cycle. Good tools for measuring intracellular...cell culture and reversible control of signalling pathways. These LIDs are generalizable, versatile and powerful...reversibly switch off and on your favourite signalling pathways (Figure 2). They are all available now at Addgene...Understanding how a protein traffics through these pathways is key to understanding, and potentially perturbing... which can then be tracked along the secretory pathway (Figure B). The RUSH system has already been used...modified sdChr- a blue-shifted channelrhodpson from freshwater green alga identified by Ed Boyden’s lab. The...for identifying previously unknown factors and pathways involved in pre-mRNA splicing in a compound screen...

  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins...Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization of a protein... Many of the proteins that enter the secretory pathway require chaperones to help with folding or post-translational...search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene...

  3. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...kynurenine pathway of tryptophan degradation. It turns out that activation of the PGC-1a1 pathway increases...protected. Effects of Activation of the PGC-1a1 Pathway Gene expression analysis of the animals’ skeletal...

  4. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...approaches. The first route involves identifying a gene/pathway to develop a drug for (aka a druggable target)....reveal individual genes or sometimes even genetic pathways that are worth investigating for drug development...’ proteins – having multiple hits or an entire pathway of possibilities greatly increases the odds of ...

  5. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...the Gβγ dimer, activating downstream signaling pathways.  This dissociation can be measured using a technique...system. Conclusions Although the importance of GPCR pathways is well known and, as a result, a target of many...collection can be used to study endogenous ligands, the pathways they activate, as well as the activation by drugs...

  6. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...need to learn much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage...finally grasped just how complicated DNA repair pathways are. I fully admit to being a DNA repair newbie...template strategies activate different HDR sub-pathways like synthesis-dependent strand annealing (SDSA...

  7. Synthetic Biology - Metabolism

    Type
    Collection
    ...to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways Efflux Pumps...Synthetic Biology plasmids for metabolic enzymes and pathways. Plasmid...

  8. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...the preferred pathway for generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing... counterpart, enabling study of human-relevant pathways (Liu et al., 2017). Beyond human relevance, yeast...

  9. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...repaired by the non-homologous end-joining (NHEJ) pathway disrupt target gene sequences (thus inactivating...breaks repaired by homology directed repair (HDR) pathways can modify the sequence of a gene (thus altering...Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab Members who worked on ...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...potential toxicity and cross-reactivity with other pathways. Since these effectors are diffusible, they’re...input patterns may also be used to engineer new pathways with sophisticated gene expression controls. Most...inducible promoters that could crosstalk with other pathways. They also reduced system leakiness and increased...

  11. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... process. The process can range from a defined pathway or reaction to a complex event such as cancer or...acquired a favorable mutation. But if the cellular pathway of interest conveys no significant survival advantage...advantage, such as a non-essential mechanism or any pathway with cellular redundancies, a screen would be required...

  12. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...metabolic engineering, the expression of enzymes in a pathway will ideally be optimized to promote generation... the shuttling of the intermediates into other pathways. In cases where a specific ratio of products is...variability between the different components. When your pathway needs precise fine-tuning of gene expression levels...

  13. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...etc., microbially or in cell-free systems. These pathways typically involve multiple steps in which a precursor...isolated afterwards. To engineer these production pathways, MoClo has been used to assemble multigene constructs...testing any number in the Level 2 assembly. These pathways can require a lot of optimization - which is why...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...cell viability and increase protein production (Schwarz et. al., 2020). Harvest and purification At its....2013.00217. PMID: 23908655; PMCID: PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, ...Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau ...

  15. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...vectors - which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...Recombination or Alternative End Joining, two alternative pathways. See (Guo et al, 2023) for a review of other targeted...to their location (e.g. in a gene in a similar pathway, a growth factor, etc.) this control is usually...

  16. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...several proteins in the DNA oxidation and repair pathways work downstream of Tet1 to restore the DNA after... S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015) Stable oncogenic silencing...coupling of methylcytosine oxidation and DNA repair pathways. Nature Communications 10:. https://doi.org/10.1038...

  17. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...breaks into the homologous recombination repair pathway. Reagents required to perform pha-1 co-conversion...researchers to quickly and easily start genome editing (Schwartz et al., 2018). To see more CRISPR tools for C.... . https://doi.org/10.1534/genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing...

  18. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...advantage of repair pathways within the cell — in some cases, the same repair pathways that were considered...transversion editors rely on a multi-step cellular repair pathway that is known for sometimes causing double-strand...

  19. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...demonstrating that this system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin... M., Nguyen, T. H., Prathima, P., Fithian, W., Schwartz, M. A., Hacohen, N., Doench, J. G., LaFleur, M...

  20. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ... will be able to better choose which genes and pathways to target in future small molecule screens. He...coworkers to test inhibitors of a variety of signaling pathways for their effects on the disease. This work will...
Showing: 41 - 60 of 188 results