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We narrowed to 61 results for: lentiviral particles

Showing: 41 - 60 of 61 results

  1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...of virus, not the individual viral particles. A single viral particle is called a virion. Finally, viral...they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not just ...many After a virion infects a cell, the single particle creates many copies of itself, sometimes up to...eliminate their replicative ability. The remaining particle, which retains the protein coat (and envelope)...genetic payload to a cell but cannot create new particles. As a bonus, this increases the carrying capacity...after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that...

  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...-studied lentivirus is HIV, which many lentiviral tools have been derived from. Lentiviruses have slightly...Unlike gamma, lentivirus can infect both dividing and non-dividing cells. Lenti particles enter the nucleus... with immortalized cell line cultures. Lentivirus Lentivirus is a type of retrovirus and shares many ... commonly used lab viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus...nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus are so closely related, ...specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering...

  3. Lentivirus ddPCR Titration

    Type
    Protocol
    ...close to zero (A01). In this protocol, the lentiviral particles are serially diluted and used to transduce...generally represents the number of infectious viral particles. Users may need to run lower or higher dilutions...Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious Titer by a Novel Droplet Digital PCR... of Lentivirus Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence... PCR (ddPCR) for lentivirus vector titration. Protocols... Protocols ddPCR Titration of Lentivirus Vectors ddPCR Titration...Assay AAV Titration by qPCR Addgene Viral Service Lentivirus Guide Virus Blog Posts Introduction This protocol...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...think I need. Unlike lentivirus, AAV requires a higher number of viral particles for efficient gene transfer...alone. If yield is important, or if the viral particles of the AAV serotype you’re working with are primarily...ethanol and dry ice bath to release the virus particles from the cells. Cell lysates are then treated ...quantifies the number of genome-containing viral particles that are present in an AAV prep. AAV samples are...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...AAV viral preps for systemic delivery of viral particles that will target the central nervous system, peripheral...

  6. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...production of viral particles, and transduction of a target cell line with those particles. Since the Env,...extra steps to ensure that particles are not sprayed into the air. For lentivirus and other BSL-2 materials...viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...humans, earning this virus a BSL-2 classification. Lentivirus (a type of retrovirus) and gamma retrovirus require...and required in BSL-2 labs. When working with lentivirus, many organizations require or recommend disposable...you have to work with BSL-2 materials, such as lentivirus. Most of this work will need to be done in a ...for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge bin covers, which...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...infectious titer, meaning the concentration of viral particles that can transduce cells, which can vary based...physical titers, meaning the concentration of viral particles containing a viral genome. While ddPCR is superior...& Kleinschmidt, J. (1999). Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes...production process.  Helper-free production Unlike lentivirus, AAV does not possess the ability to replicate...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...sequence all the DNA packaged inside the viral particles (Figure 1). Briefly, packaged DNA is extracted...recombinase-independent recombination occurs in 0.1-0.6% of AAV particles in a given viral prep. With this in mind, we recommend....org Find Ready-to-Use AAV Find Ready-to-Use Lentivirus Browse Viral Plasmids ...

  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...a brief overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes...different AAV vectors into a brain region, the AAV particles will infect neurons at different ratios, making...and may be a form of transcytosis where the AAV particle is taken up on one side of the cell and released...cell. While there is always a risk that viral particles will diffuse long distances within the extracellular...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...traditional dye-based neuronal tracing reagents, AAV particles require receptor-mediated entry into the cell....Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service ...Ready-to-use Virus for Your Research Visit Our Lentivirus Guide Page ...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...sequences are not efficiently packaged into lentiviral particles. See below for options if the recombinant...gel ( see restriction digest protocol here ). Lentiviral plasmids can recombine between their LTRs resulting...

  12. Lentiviral Vector Guide

    Type
    Guide
    ...Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...safety. For a summary of all lentiviral plasmid components, see the Lentiviral Plasmid Elements table . In...different generations. Lentiviral Generations First-generation First-generation lentiviral plasmids are largely...Second-generation Second-generation lentiviral plasmids greatly improved the safety of lentiviral vector production by...between second- and third-generation lentiviral plasmids. Lentiviral Vector Production Cloning Cloning your...

  13. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...determine viral tropism—the type of cells the viral particles infect. This can direct your biosensor to specific...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

  14. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...frequency and the viral titer (the number of viral particles capable of infecting a host cell) can be tuned...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...

  15. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...gamma-retroviruses may not be efficiently packaged by lentiviral packaging systems, and vice versa. Gamma-retroviral... 293T (HEK293T) cells (Figure 3A), similar to lentiviral packaging methods . The three plasmids described... change, the supernatant containing the viral particles is removed and stored or centrifuged to concentrate...BioRender.com. Viral Vector Integration Much like lentiviral vectors, gamma-retroviral vectors integrate at...types and enhances the stability of the viral particles. However, VSV-G is toxic under long-term, constitutive...to make stable cell lines. Like their popular lentiviral counterparts, gamma-retroviral vectors offer ...

  16. CRISPR Guide

    Type
    Collection
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in vivo ...

  17. Plan Your Experiment

    Type
    Guide
    ...produce the viral particles. Then you transduce your cells using the viral particles before you continue...production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular option for producing...types like primary cells. For CRISPR delivery, lentiviral and adeno-associated virus (AAV) vectors are ...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...we don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide...CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages...advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to use in the lab, but ...

  18. Molecular Biology Reference

    Type
    Guide
    ...to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect...service offers select ready-made AAV and lentiviral particles. Visit our viral service page to learn more...

  19. CRISPR Guide

    Type
    Guide
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in vivo ...
Showing: 41 - 60 of 61 results