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We narrowed to 65 results for: lentivirus production

Showing: 41 - 60 of 65 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...transcription, and integration needed for the production of lentiviral particles. It’s capable of inducible...posts Read our viral vector blog posts Read our lentivirus guide Resources on the Addgene website Browse...

  2. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...neuroinflammation and anxiety by modulating the production of the pro-inflammatory cytokine interleukin ...closed arm (safe). Mice with higher IL-1 beta production spent more time in the open arm and were less...floating. In the same paper, the authors used lentivirus to introduce an shRNA to downregulate expression...

  3. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...PMC2957237. Okada, T. (2013). Efficient AAV Vector Production System: Towards Gene Therapy For Duchenne Muscular...? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Saccharomyces cerevisiae, as a key host for the production of their products, including fermented beer and wine, ...use the library, the authors packaged it into lentivirus and used the resulting lentiviral library to ...applications. First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed...ingredients, and recombinant proteins. In order to keep production yields high and costs low, and to deal with the...dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing... system has a much larger cargo capacity than lentivirus and is capable of efficiently creating stable...

  6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral...this approach could permit some viral protein production, so researchers are also interested in precisely...only after stable expression of Cas9/gRNA. Viral production is higher in this model than in latent HIV-1 ...

  7. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...red pH indicator) because the buildup of waste products could be suboptimal for cell growth. This conditioned...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...precursors, biofuels, and natural products without the undesirable side products generated during chemical synthesis...these molecules. To make large amounts of pure product in an affordable way, researchers test many different... resistance through gene stacking, increasing production of a protein of interest, and improving the nutrient...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors.” ACS Synthetic...

  9. Lab to Office Culture Shock

    Type
    Blog Post
    ...on my technical skills with cloning and lentivirus production, I didn’t really start to recognize my transferable...

  10. Hot Plasmids - November 2023

    Type
    Blog Post
    ... to target multiple delivery systems, such as lentivirus and MMLV gag, to specific cell types. It can ...cell therapy more accessible by simplifying the production process and could even enable the creation of...

  12. Plan Your Experiment

    Type
    Collection
    ...more likely to result in a non-functional protein product. Be aware that there could be downstream translation...more information about viral vectors and their production, see our viral vector guides . Lentiviral vectors...into your viral vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ...plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9...

  14. Lentiviral Vector Guide

    Type
    Guide
    ...plasmids available at Addgene. Lentivirus Versus Gamma-Retrovirus Lentiviruses and gamma-retroviruses fall...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...the potential for replication-competent lentivirus production — that is, creation of viral particles that...lentiviral components, generations, lentiviral production, and common uses. Guides...Lentiviral Vectors Lentiviral Generations Vector Production Integration Pseudotyping Common Uses Safety Resources... Resources Plasmid Elements Glossary Lentiviruses are a type of retrovirus and belong to the Retroviridae...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ....aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with... restriction site and a linker region. The PCR product is then ligated into the MCS/SacI site in pUCXKT...occur in this system if a small contaminating PCR product formed from primer dimers and containing the missing... travels along the mRNA to produce the protein product. The system, cleverly termed TRICK, for Translation...the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block...Methods, Georgieva et al describe the rational production of a new series of epitope tags, inntags (innocuous...of interest (POI). This technique requires the production and expression of fusions between the POI and...

  16. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...available at Addgene. Gamma-Retrovirus Versus Lentivirus Lentiviruses and gamma-retroviruses fall under the Retroviridae...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...about gamma-retroviral components, retroviral production, and common uses. Guides...Guide Jump To: Gamma-Retroviral Vectors Vector Production Integration Pseudotyping Common Uses Safety Resources...containing only the necessary packaging genes, while lentiviruses also contain accessory genes specific to each...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral...

  17. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...sites including both the edit rate and the repair product sequence. Targeted amplicon sequencing doesn’t ... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

  18. CRISPR Guide

    Type
    Collection
    ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...G-to-T:A base editors with higher efficiency and product purity. Science Advances . 3 (8), eaao4774. PMID...

  19. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...their production and purification that yield high titers (see also AAV protocols for production, purification...tracing compounds either produce a colorimetric product or are fluorescent. While dyes usually spread unidirectionally...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is...
Showing: 41 - 60 of 65 results