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We narrowed to 275 results for: mel

Showing: 41 - 60 of 275 results

  1. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...will ligate. Subsequent cycling through rounds of melting and annealing allows linear amplification of the...for certain, but may have to do with the slight melting of the 5 ́-terminus during the reaction. This “...incubating the ligation at a temperature near the melting temperature (Tm) of the DNA strands. This selectively...Electrochemiluminescent assay for detection of extremely rare mutations based on ligase reaction and bead...

  2. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...the experts in the CRISPR/Cas field, have been extremely helpful for scientists that are looking for more...on “Making Your Own Competent Cells” has been extremely popular, generating many additional questions....and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's...

  3. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...unmatched customer service that is consistently timely, informative, friendly, and professional Addgene..., Lianna, Luke, Marcy, Mary, Max, Maya, Meghan, Melina, Meron, Michael Lo., Michael Le., Michel, Michelle...Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey, Randall, Ray, Rebecca, Renee, Shannon...

  4. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...adventure. Many thanks to our guest writer, Pamela J. Hines, PhD! Pamela J. Hines is a Senior Editor at Science...This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...This post was contributed by Manon Eckhardt and Melanie Brewer from the QBI Coronavirus Research Group ...to our guest bloggers Manon Eckhardt (left) and Melanie Brewer (right) from the University of California...California, San Francisco. Manon Eckhardt and Melanie Brewer are on the Science Communications Team at QBI UCSF...

  7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...factors (which repair those breaks) makes Cas9 extremely effective as a genome editing reagent. This interplay...2014) have shown that this technique supports extremely high frequencies of genome cutting and, when donor...DNA is included in the nucleofection reaction, extremely high frequencies of HDR. The detailed protocol...

  8. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...analysis performed in experiment 1. Use camelCase: In camelCase, each subsequent word in a file name after... more than one word should be named in the camelCaseFormat since spaces between each word can make it ...

  9. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...microbiome species such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed...antibiotic treatment. To facilitate these studies, Pamela Silver’s lab developed a synthetic mark and recapture.... PubMed Central PMCID: PMC6467691. Takahashi, Melissa K., et al. "A low-cost paper-based synthetic biology...

  10. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...rings, dilution cloning into 96-well plates, is extremely time- and resource-consumptive, since only wells...densities for selection). A 2% agarose solution is then melted (and thereby sterilized) by microwaving and brought...left covered (to avoid drying out) while 3 ml of melted agarose are mixed with 3 ml of 2X DMEM in a 50 ...

  11. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... questions, and (most importantly) decide on a timeline. My experience is that tasks often don’t get done...discussion can be confidential. Finally, provide timely feedback. We often associate feedback with end-...feedback should be given throughout the year and in a timely manner. If someone’s performance or behavior is...

  12. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...one developed for Zaire ebola virus (EBOV) are extremely useful, allowing researchers to study aspects ... systems, such as the one described here, are extremely useful research tools. In addition to the reduced... each plasmid transfected into cells are also extremely important. For example, particular ratios of plasmids...

  15. Technical Design of a Western Blot

    Type
    Blog Post
    ...agent than BME and has a less… distinctive smell. BME smells like rotting eggs but has a longer shelf life...decision is probably already made: gradients are extremely difficult to handcast, so unless you have an expert...

  18. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...cytochrome P450 genes discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are...mediate steroid hormone biosynthesis in Drosophila melanogaster." Molecular and cellular endocrinology 215.1...

  20. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...you during the request stage, before you order.  Timeline Once your order is placed, we estimate it will...and QC assays to be completed and reviewed. The timeline therefore will be as follows:  Request review:...
Showing: 41 - 60 of 275 results