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Showing: 41 - 60 of 397 results

  1. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    ...have a name. Last month, we collected suggestions for names and we’ve recently chosen four names as finalists...friend finally has a name! We thank you for your participation in suggesting names and voting in our poll...meaningful names…. Watch the video to find out the final four! (Note: For cases where the same name was submitted...love the way the names Aavery, Addy, and Cappy play on viral vector terms. And, the name Kendall has a special...

  2. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...scripts. Naming conventions Give every experiment a number: Numbers are a short, easy way to name files ...a given day. For example, instead of naming a file “output” name the file “1_output” so that you know ...not separated by spaces. File names with more than one word should be named in the camelCaseFormat since... file system in the computer and is just another name for a folder. For instance, your Desktop directory.... This can be a simple .txt document. Record the name, date, and location of all analyses (see above)....camelCase: In camelCase, each subsequent word in a file name after the first word begins with a capital letter...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...

  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...Description Plasmid Name Use a descriptive plasmid name and match the name as it appears in its publication... it in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of your PI and send...chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin...with the following information: Addgene account username. In order for the plasmids to appear in your Addgene...Plasmids, we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences...Hold for Publication or Distribute pending QC. The name of the Principal Investigator and Organization where...

  4. New and Upcoming Viral Vectors - December 2019

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    Blog Post
    ... both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1...inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, ...Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities as neurotransmitter...AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE 104493...offering medium apparent affinity. Plasmid Serotype Name 113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h 113049...has been packaged into AAVrg. Plasmid Serotype Name 106174  AAVrg pAAV.hSynapsin.SF-iGluSnFR.A184S...website to access them! DREADDs Plasmid Serotype Name  44361  AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry   ...

  5. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...sequential IDs.   Name – we encourage the use of descriptive plasmid names and using the exact names listed in...Plasmid to this Article” (left) and entering a plasmid name,plasmid type, and plasmid description/experimental... purpose (right). On the article page, enter the name, type, and description of each plasmid associated...as explained below: Pro Tip! Add all the plasmid names and descriptions first before entering the data....the intended purpose of the plasmid. Once you've named and described all of the plasmids in your deposit... not appear for empty backbones Please enter the name of any gene contained within your insert (or targeted...auto-populated list, try entering the gene's Official Full Name or one of its "Also known as" aliases in the Gene...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362...inventory for the entire collection! Plasmid Serotype Name Depositor 27056 AAVrg pAAV-Ef1a-DIO EYFP Karl ...through the mDlx enhancer element. Plasmid Serotype Name Depositor 75470 AAV1 pAAV-CAG-FLEXFRT-ChR2(H134R...and promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry..., is also available in AAV1. Plasmid Serotype Name Depositor 69570 AAV1 pAAV-EF1a-N-CretrcintG Connie...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO mCherry 83900 AAVrg...-GFP-Fishell-1 Biosensors Plasmid Serotype Name 83899 AAVrg pAAV-mDlx-GCaMP6f-Fishell-2  ...

  7. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... concentration lies within the dynamic range of the curve. The dynamic range is the linear span between...concentrations that the curve can accurately measure.  Dynamic range depends on a number of factors, including...instrument being used to measure the response. The dynamic range needs to be wide enough to cover all possible...; however, it should not be too broad. A broad dynamic range covers a wider span of concentrations but...measurements for the unknown sample fall outside of the dynamic range of the curve, then the assay is invalid. ...linear portion of the graph. It does restrict the dynamic range of your standard curve, so if your sample...ahead of time to see what factors may impact the dynamic range of the assay. Try to minimize these.  Review...

  8. Anatomy of a Plasmid Page at Addgene

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    Blog Post
    ...publication. Next up, we have the plasmid name: This is the name given to the construct by the depositing...other plasmids with similar names in the collection, but you can use this name to find this plasmid from... I know who to acknowledge? Depositing lab: The name of the Principal Investigator (PI) in whose lab ...lab the construct was made. The depositor name links to a page listing all of all this PI’s plasmids that...optimal growth temperature are listed, as well as the name of the strain.  Most Addgene plasmids are maintained...

  9. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...you started! Dear Dr./Professor [last name], My name is [full name]. I am a [type of scientist] who recently...earned my PhD from the Department of [name of the department] at [name of the university].During my thesis...writing to apply for [title of the position] at [name of the company/organization]. I am a [adjective ...talks at conferences and taught for five years at [name of the university]” Include keywords from the job...

  10. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ...happy to learn that many DNA-based reagents are named for soup ingredients! From pSOUP to mCherry, satisfying...know this best, as most fluorescent aptamers are named for a fruit or vegetable! What is an RNA aptamer...aptamers permit the study of intracellular RNA dynamics. Aptamers are 10-100 base nucleic acid oligos ...using Spinach2 showed its ability to track RNA dynamics in a model of Fragile-X tremor/ataxia syndrome...been used to investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017)...For dessert (last but not least), the only fruit-named aptamer, Mango, binds to multiple derivatives of... Jaffrey. "A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing RNA."...

  11. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ...the vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472...Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype Name 75033...manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA 121675...pAAV-EF1a-Flpo Calcium sensors Plasmid Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1...

  12. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post
    ...scientists need a protease reporter that has a large dynamic range and high brightness. The Shu Lab from UCSF...fluorescence range to better study the spatio-temporal dynamics of apoptosis in living animals. To do this they... by flipping a single β-strand of GFP, hence the name FlipGFP. FlipGFP increases fluorescence 100-fold...Shu Lab used FlipGFP to track the spatiotemporal dynamics of apoptosis during embryo development. GFP fluorescence... a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter achieves...scientists to observe both the spatial and temporal dynamics of apoptosis both in vitro and in living organisms...

  13. Troubleshooting and Optimizing a Western Blot

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    Blog Post
    ...sample or protein of interest to understand the dynamic or working range of a given reagent (usually an...protein concentrations are within the antibody’s dynamic range. Created with biorender.com   Alternatively...recommended dilution. If the antibody dilution’s dynamic range encompasses your protein’s expected concentrations...dilution until your protein dilutions are in the dynamic linear range (i.e., expression decreases as protein....     Figure 3: Testing the antibody’s dynamic range. A) The protein dilutions, representing expected... concentrations, are all within the antibody’s dynamic range. B) The protein dilutions, representing expected... concentrations, are outside of the antibody’s dynamic range. Specifically, the 1:2, 1:1, and No dilution...

  14. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ... creating the SunTag system. The SunTag system, named after the "stellar explosion SUperNova", is a synthetic...contains a nonfunctional truncated version of the kanamycin resistance gene (missing the first two codons)...pUCXKT will therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False... allows the study of loci proximity and of the dynamic interactions between intra- and inter-chromosomal...LOC8R-PAGFP provides a novel strategy for assaying the dynamics of different mitochondrial subpopulations within... Ed Boyden’s lab. The enhanced sdChr construct, named CheRiff, exhibits high light sensitivity and fast...changes reduced system leakiness and increased the dynamic range, resulting in more user-friendly and tunable...

  15. TREAT-AD Plasmid Collection

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    Collection
    ...You can search or sort this table based on plasmid name, gene/insert, and more. ID Plasmid Gene/Insert Vector...

  16. MAPK Plasmids

    Type
    Collection
    ...MAPK Plasmids (organized by gene) Gene Alternate Names MAPK1 ERK, ERK2, ERT1, MAPK2, P42MAPK, ...

  17. Technical Design of a Western Blot

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    Blog Post
    ...broad dynamic range that typically spans 2–5 orders of magnitude, which includes a wide dynamic range ... may be light-sensitive. They also have a poor dynamic range, meaning that if your blot contains one protein...for multiplexing. They have a ten-fold greater dynamic range than chemiluminescence enzymes, making it...developer. Film is highly sensitive but has a limited dynamic range, which means it may take multiple exposures...background Chemiluminescence reporter Smaller dynamic range; no multiplexing Protein-based blocker...background Chemiluminescence reporter Smaller dynamic range; no multiplexing Wet transfer Increased...

  18. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post
    ...to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek mythology) switches...PA-FP is the naturally occurring Dronpa protein (named after dron, a ninja term for vanishing, and pa, ...trafficking, as well as organelle trafficking and dynamics (fission, fusion, etc). For example, by engineering... was able to visualize and quantify the fusion dynamics of mitochondria in healthy and apoptotic cells...at their fingertips the tools to probe cellular dynamics and function with extraordinary molecular detail...Mariusz, et al. "Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows...

  19. Improved Plasmid Maps Powered by SnapGene

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    Blog Post
    ...resistance gene, you’ll find the full name of the gene, the name of its gene product, and a description...cut the given sequence. The table includes enzyme names, the locations where the enzymes will cut (in basepairs...the plasmid sequence. The table includes feature names, locations, sizes, colors used to indicate them ... nucleotide sequence. The table includes primer names, sequences, binding site locations, lengths, and...

  20. dTAG - You're it!

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    Blog Post
    ...for dTAG molecules and any questions to Behnam Nabet (behnam_nabet@dfci.harvard.edu), Nathanael Gray (nathanael_gray...This post was contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer ...et al.), and in vivo studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber... the dTAG technology. Follow him on twitter @behnamnabet.   References Nabet et al. The dTAG system for...
Showing: 41 - 60 of 397 results