We narrowed to 104 results for: pCas

1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   ...protein The MLV Gag protein fused to a Flag-tagged SpCas9 is incorporated into Nanoblades, just like the ...these variants are fused to the MLV Gag in place of SpCas9. gRNA A gRNA targeting a DNA sequence of interest...

2. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   ...well characterized than the conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes...been shown to exhibit more limited activity than SpCas9 (2). In many cases this is due both to lower relative...

3. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ...Enhance CRISPR Targeting Specificity with eSpCas9 and SpCas9-HF1 See How Cpf1 Stacks Up to Cas9 and NgAgo...

4. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   ...multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the ICE software...configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency (percentage...

5. CRISPR 101: Validating Your Genome Edit

   Type

   Blog Post

   ... for reduced off target editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options...

6. CRISPR 101: Cytosine and Adenine Base Editors

   Type

   Blog Post

   ... deaminase portion of the base editor to create SpCas9 base editors with editing windows as small as 1...available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM site Chen et al., 2023 Td-CBE...

7. 22 Hot Plasmid Technologies from 2014

   Type

   Blog Post

   ...target sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping... From the lab of Yonglong Chen, pCS2-3xFLAG-NLS-SpCas9-NLS is a Cas9 expression plasmid that was used ...

8. Editor's Choice, July 2016

   Type

   Blog Post

   ...compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other benefits...

9. pSiM24: Simplifying Plant Genetic Engineering

   Type

   Blog Post

   ...2 and 6 kb smaller than commercially available pCAMBIA and pKYLX vectors, respectively, making it ideal...

10. Mapping the 4D nucleome with CRISPR/Cas9

    Type

    Blog Post

    ...create a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...

11. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    ...nickase experiment Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation...

12. We're Updating Our Hold for Publication Policy

    Type

    Blog Post

    ...published-paper-celebration checklist as champagne or cupcakes, or maybe the lab member originally responsible...

13. Hot Plasmids and Viral Preps - July 2021

    Type

    Blog Post

    ...editing in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and...

14. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    ...The PAM Requirement and Expanding CRISPR Beyond SpCas9 The closer your edit is to the PAM site, the higher...

15. New and Upcoming Viral Vectors - December 2019

    Type

    Blog Post

    ...collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO...

16. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    ...887 amino acids in length, NgAgo is shorter than SpCas9 (1368 aa), SaCas9 (1053 aa), or Cpf1 (1228 or 1307...

17. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    ...promoter directs broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs...

18. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    ...different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to...

19. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    ...create a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...

20. What's New in CRISPR - November 2021

    Type

    Blog Post

    ...with these modified gRNAs is comparable to that of SpCas9. The group also confirmed that the Cas12f1 and ...