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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 105 results for: pcas

Showing: 41 - 60 of 105 results

  1. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...well characterized than the conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes...been shown to exhibit more limited activity than SpCas9 (2). In many cases this is due both to lower relative...

  3. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the ICE software...configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency (percentage...

  5. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... deaminase portion of the base editor to create SpCas9 base editors with editing windows as small as 1...available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM site Chen et al., 2023 Td-CBE...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...target sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping... From the lab of Yonglong Chen, pCS2-3xFLAG-NLS-SpCas9-NLS is a Cas9 expression plasmid that was used ...

  7. Editor's Choice, July 2016

    Type
    Blog Post
    ...compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other benefits...
Showing: 41 - 60 of 105 results