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We narrowed to 416 results for: plasmids 101

Showing: 41 - 60 of 416 results

  1. Fluorescent Proteins 101: Luciferases

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    Blog Post
    ...collection page Promega Plasmid Collection Fluorescent Proteins 101 ebook ... plasmids 101...Engineered   677 61 AkaLumine ATP No Common uses for plasmids expressing luciferase Luciferase reporter assays... partnered with Promega to offer their Promega Plasmid Collection, including tools using NanoLuc®, NanoBiT...Enabled by NanoLuc® Luciferase Fluorescent Proteins 101: Introduction to FRET Resources on Addgene.org ...

  2. Plasmids 101: Blue-white Screening

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    ...This post is part of our ongoing Plasmids 101 series. Plasmids 101 will provide you with an overview ... the Plasmids 101 posts here. Now that we have covered antibiotic selection here at Plasmids 101, we can...α-complementation plasmid. This way you can quickly and easily identify colonies that not only have your plasmid (antibiotic...rescued by genes supplied on the transformed plasmid. Such plasmids may contain genes that allow for the use...Use Yeast Two Hybrid Systems Browse All of Our Plasmids 101 Posts Resources on the Addgene Website Choose... able to select for colonies that contain your plasmid is a great start when cloning, but how about being...being able to choose those that contain plasmid with an insert? Blue-white selection is a widely used ...

  3. Adeno-associated virus (AAV) Plasmids

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    ...AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids 101-Viral...AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV Plasmids Adeno-associated viruses (AAV)...with the plasmid that encodes the viral genome (typically called cis -plasmid or transfer plasmid). Helper...Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for AAV packaging...table based on plasmid name, capsid, and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table... Viral Plasmids Adeno-associated Virus (AAV) Plasmids Adeno-associated Virus...flexibility. AAV Packaging Plasmids The following tables contain a list of packaging plasmids that are useful to...

  4. Plasmids 101: Antibiotic Resistance Genes

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    ... presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing cell...to maintain the plasmid. Adding an antibiotic resistance gene to the plasmid solves both problems at once...Additional Resources on the Addgene Blog Read other Plasmids 101 Posts Learn How to Use Blue White Screening ...Biology Reference Pages View our Plasmid Protocols Find Your Perfect Plasmid Backbone ...think about how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient... which cells successfully received the correct plasmid, scientists would spend hours to days trying find...cell must replicate the plasmid in addition to its own chromosomal DNA, costing additional resources to ...

  5. Cre-Lox and Other Site-Specific Recombinases

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    ...Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids Other Recombinase Plasmids Recombinase-Dependent...with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout... vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Request service for more options. Addgene blog Plasmids 101: Cre-lox Cre-ating New Methods for Site-specific...Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Search...Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...

  6. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

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    ...letter of a plasmid name and simply denotes that the object is a ‘plasmid’. “p” is for plasmid. pXXXXX-XXXX...about plasmid features and tips you can use in the lab, check out more posts in our Plasmids 101 series...There are no universal rules for naming plasmids but here are some good guidelines to follow in order...people can quickly and easily identify what your plasmid contains and other important information. Tip:...Backbone name Include the empty backbone name in your plasmid name. This simple piece of information can often...important details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial...  Include information about the insert in your plasmid name. This is often a 3-6 letter representation...

  7. Plasmids 101: Methylation and Restriction Enzymes

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    ...desired mutation into your plasmid of interest by PCR generates mutated plasmids with no methylation (there...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular... for purposes other than cloning or confirming plasmids. It turns out that restriction enzymes are one...difficult to interpret or lead you to conclude your plasmid is not correct! Conversely, enzymes such as DpnI...methyltransferases in the PCR reaction). The template plasmid, on the other hand, should be derived from a dam...methylated adenines in any GATC sequences found in the plasmid. When the PCR products are digested with DpnI, ... is destroyed leaving behind a pool of mutated plasmids which can later be verified by Sanger sequencing...

  8. Viral Vectors 101: Virus Safety

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    ...transfer plasmid), the envelope (Env) gene on a separate plasmid (envelope plasmid), and the polymerase ...different plasmids. For a retroviral system this looks like the genetic cargo in one plasmid (the transfer...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection... transfection of all three plasmids into a producer cell line, such as HEK-293T, production of viral particles... and Pol genes are not encoded in the transfer plasmid, the component that physically integrates into ... retroviral packaging components onto distinct plasmids for safety.    Viral cargo-specific safety...services Addgene Biosafety Guide Addgene Viral Plasmids Which Type of Virus Should I Use? Resources...

  9. Viral Vectors 101: Viral Vector Elements

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    ...lentiviral plasmids Addgene's most popular retroviral plasmids Addgene's collection of AAV plasmids Addgene...split across several plasmids (often three), for safety purposes. The primary plasmid with the delivered...often a gene) is called the transfer plasmid while the other plasmids with packaging or replication components...of adenovirus plasmids Resources on the Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral...For safety and production reasons, multiple plasmids with many components are used to package lab grown...can be intimidating at first to look at so many plasmid maps and protocols! In reality, there are many ...components are called helper plasmids. Below, we’ll breakdown the vector components first by virus and then...

  10. Plasmids 101 eBook 4th Edition

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    Blog Post
    ...popular eBook, Plasmids 101, is now available!   Plasmids 101 covers everything from plasmids parts and ...word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia; they go to Addgene...

  11. Plasmids 101: Golden Gate Cloning

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    ...Check out more Plasmids 101 Posts! Check out Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression... plasmids 101...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...Entry DNA overhangs may be present in the original plasmid (Option 1) or added using PCR-based amplification...fragments to create TAL effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type... a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy not only makes...makes it easy to create a single gRNA-expressing plasmid, but it can also be adapted to express multiple...

  12. Plasmids 101: Common Lab E. coli Strains

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    ...Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page!     ...mutation Improves plasmid yield F Host does (F') or does not (F-) contain the fertility plasmid. A low copy-number...and general DNA repair Reduces plasmid recombination, increases plasmid stability recBCD Exonuclease...others are model organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region...fhuA::IS2 DB3.1 Streptomycin  HB101 derivative. For propagating plasmids expressing the ccdB gene (important...Nov 14, 2017. You've worked hard designing your plasmid – you carefully selected the optimal promoter for...labor-intensive process of replicating your new plasmid: the Escherichia coli bacteria. It’s hard to count...

  13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...every isolated plasmid sample and normalize each to fall within a 10 fold range. Plasmids that don't meet...A broader view of the whole plasmid To confirm the sequence of the plasmid, we examine three things: ...expected and do not affect plasmid function, we will update information on the plasmid’s page. Sometimes we’re...’re missing a piece of the plasmid There are regions of some plasmids that are particularly difficult ...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are ...bottom of the plasmid page. Viral preps and pooled libraries In addition to regular plasmid sequencing,...sequence all plasmids in the pool but instead selectively sequence the variable portion of the plasmid in the...

  14. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...233–247.e17 . https://doi.org/10.1016/j.cell.2016.08.056 Find plasmids from this publication at Addgene...chicken embryo. BioRxiv: https://doi.org/10.1101/135525. Find plasmids from this publication at Addgene. ...cytosine demethylation in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation...Tet1CD, is available from Rudolf Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure...Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1...experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   Leah Schwiesow...510–517 . https://doi.org/10.1038/nbt.3199 Find plasmids from this publication at Addgene. Klann TS, Black...

  15. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    ...with some plasmids. You just need three plasmids to start making AAV:  the packaging plasmid which contains... transfer plasmid an AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ...of an AAV transfer plasmid. Parts shown in green are key elements of an AAV plasmid. Parts shown in grey... elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal ...transfer plasmids have a promoter, but this element isn’t required when the transfer plasmid harbors non-coding...Transfer Plasmid video!   Now you’ve got a basic understanding of some common AAV plasmid elements!...structural and packaging genes, the adenoviral helper plasmid which contains the proteins needed for the virus...

  16. Plasmids 101: Terminators and PolyA signals

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    ...Download our Plasmids 101 eBook to get all the background you need to start working with plasmids! What are...Techniques Read all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just...Plasmids designed to express genes in a given host cell type are generally broken down into two broad...based on the functional elements they contain. Plasmid DNA in both prokaryotic and eukaryotic systems ...Rho-dependent terminators are not usually employed in plasmid-based expression systems, so these will not be ...the end.  Nearly all common bacterial expression plasmids use Rho-independent terminators, which include...termination with polyadenlyation. Mammalian expression plasmids are primarily used to create mRNA and the commonly...

  17. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...Antibiotic Plating Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Antibiotic Resistance...Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...resistance DNA plasmids are traditionally grown in bacteria as a way to produce more of the plasmid, isolate...isolate individual plasmids, or express a gene contained in a plasmid. During the propagation and/or transformation...receive a copy of the plasmid or will lose it during division. These non-plasmid-containing bacteria can...Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your...your experiment — to grow, express, or isolate plasmids. The presence of an antibiotic resistance gene...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...comparison of an shRNA plasmid sequence and an miRNA plasmid sequence. (A) Plasmid sequence from pLVUHshp53...for an Old Favorite CRISPR 101: Targeting RNA with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let's....   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important steps: ...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence...shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is meant...Let's Go! Browse All Plasmids 101 Posts Additional resources on addgene.org Mammalian shRNA Tools for... occur via transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different...

  19. Plasmids 101: E. coli Strains for Protein Expression

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    ...for More Plasmids 101 Articles?  Find them all here: http://blog.addgene.org/topic/plasmids-101   ...In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli...your expression plasmid from these strains is not recommended as these auxillary plasmids may be isolated...control such as the pLys plasmid, which suppresses basal T7 expression. The pLys plasmid contains a chloramphenicol...arabinose-inducible plasmid will not express in an IPTG induction strain for example, nor will a p15 plasmid be compatible...collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region... plasmids 101...

  20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...commonly performed by transfecting cells with a plasmid, such as PX459, which encodes Cas9 and contains...chosen for both Cas9 and gRNA expression       Plasmid DNA may be incorporated into the genome     Off-target...targets with similar efficiency as compared to plasmid-based expression of Cas9/gRNA and can be used for... as CMV or EF1A promoters found in many CRISPR plasmids) are not expressed. Because this method does ...fluorophore tagged dCas9 may require the use of plasmid or viral-mediated delivery. You’ve decided to...and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab...experimental systems that may not be amenable to plasmid based methods. One of the most common techniques...
Showing: 41 - 60 of 416 results