We narrowed to 85 results for: psin

1. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   ...very high, ubiquitous expression, while the human synapsin 1 promoter is neuronal specific (Haery et al.,...

2. The Importance of High Titer for AAV Transductions

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   ...receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are required for the...

3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   ...method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator...

4. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...they wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can...

5. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ... also successfully regulated expression of channelrhodopsin-2, commonly used in optogenetics, opening ...

6. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-...

7. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   ... purified via streptavidin pulldown. Following trypsin digest, PPIs are identified via mass spec analysis...

8. Hot Plasmids - October 2020

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   ...a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now available as AAV5. Find the AAV...

9. Plasmids 101: FLEx Vectors

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   ...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...

10. Common Injection Routes in Mice

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    ... place the needle in the correct vein without collapsing it. Retro-orbital injections are also thought...

11. Cloning Mammalian Cells with the Agarose Method

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    ...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...

12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...

13. Hot Plasmids Spring 2024

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    ...they determined that an A122D mutation in the rhodopsin domain conferred several new improvements. The...

14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    ... M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range...

15. Quick Guide to Near-Infrared Fluorescent Proteins

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    ...blue-light controlled optogenetics, such as channelrhodopsins and tools based on light-oxygen-voltage (LOV...

16. Hot Plasmids: Spring 2025

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    ...to express either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the...

17. Viral Vectors 101: AAV Variables That Matter

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    ...Selective promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or...

18. Technologies Enabled by NanoLuc® Luciferase

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    ...intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light...

19. Brain Initiative Collection

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    ...optogenetics. Human Synapsin (hSynapsin) promoter Edward Boyden 107712-AAV9 pAAV-hSynapsin-FLEX-soCoChR-GFP... Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell...AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and ...pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics....pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted...Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1...

20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...short expression times, where as others, such as hSynapsin, are relatively weak and may require longer waiting...