...containing plasmids for expressing different types of rhodopsins and sensors as well as references and links to...

...very high, ubiquitous expression, while the human synapsin 1 promoter is neuronal specific (Haery et al.,...

...receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are required for the...

...they wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can...

...method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator...

... also successfully regulated expression of channelrhodopsin-2, commonly used in optogenetics, opening ...

... purified via streptavidin pulldown. Following trypsin digest, PPIs are identified via mass spec analysis...

...a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now available as AAV5. Find the AAV...

...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-...

...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...

...optogenetics. Human Synapsin (hSynapsin) promoter Edward Boyden 107712-AAV9 pAAV-hSynapsin-FLEX-soCoChR-GFP... Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell...AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and ...pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics....pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted...Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1...

...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...

... place the needle in the correct vein without collapsing it. Retro-orbital injections are also thought...

...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...

... M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range...

...they determined that an A122D mutation in the rhodopsin domain conferred several new improvements. The...

...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps... microbial opsins to be adopted as an optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from...Find optogenetics plasmids such as microbial opsin and optical switch tools to measure and manipulate...Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch Plasmids Background Optogenetics...light. Many of these plasmids encode for microbial opsins, which are membrane channels and pores derived ...cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified to increase...anions into or out of the cell. When using these opsins as a tool to control neuronal activity, scientists...

...111262 pAAV-hSynapsin1-axon-GCaMP6s Syn axon-GCaMP6s none Constitutive 1 Tian 112000 pAAV-hSynapsin1-axon-GCaMP6f...HT Promoter CAG CaMKIIa Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent... none Constitutive 1, 9, rg* Fyhn 172921 pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 Syn axon-jGCaMP8s mRuby3...mRuby3 Constitutive 1, 9 GENIE 172923 pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 Syn axon-jGCaMP8f mRuby3... none Constitutive 1, 9, rg* Fyhn 172922 pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 Syn axon-jGCaMP8m mRuby3... Constitutive 1, 5, 9 Kim , GENIE 111261 pAAV-hSynapsin1-axon-GCaMP6m Syn axon-GCaMP6m none Constitutive...-GCaMP6f none Constitutive 1 Tian 112005 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 Syn axon-GCaMP6s bicistronic...

...blue-light controlled optogenetics, such as channelrhodopsins and tools based on light-oxygen-voltage (LOV...

...intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light...