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We narrowed to 63 results for: sgrna vector

Showing: 41 - 60 of 63 results

  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells...gift from Feng Zhang and is described in Improved vectors and genome-wide libraries for CRISPR screening....is described in Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench...Doench & David Root and are described in Optimized sgRNA design to maximize activity and minimize off-target...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure ...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...expression vectors with various promoters suitable for in vivo expression, and/or produce vectors to make...prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen et al 2013) allow...donor vectors containing mAID and either Neo or Hygro resistance markers with CRISPR/Cas vectors targeting...constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides against each of... interest and cloned into provided destination vectors using low-cost, one-pot golden gate cloning that...The library was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses... Jacks Lab constructed several GMAP-compatible vectors for a wide variety of biological applications. ...

  4. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ... arms for your gene of interest into the FP-SEC vector using Gibson assembly. This construct will serve...3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... existing transposon and vector systems and creating an all-synthetic vector that included only the elements...perform genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of... into a destination vector in a predefined order. Finally, the destination vectors are used with Multisite... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...step, compatible Level 0 vectors are directionally assembled into a Level 1 vector creating a single transcriptional...of interest into the pCoofy vectors, select the appropriate pair of vector and gene primers from Table...homology between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence ...

  6. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...impact of off-target mutations, scientists are using sgRNA for selection and optimization. In addition, the...control the CRISPR system activity.  Using an AAV vector may help with size limitations. In addition, non-viral...

  7. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...many software tools predict off-target effects of sgRNAs using computational methods. They identify possible...mismatches based on the sequences of the genome and sgRNA. This is a good starting point for most researchers...and TALENs using integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175-178. PubMed...

  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...into distinct expression vectors using the enzyme BbsI. These expression vectors each contain Type IIS restriction...Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt Cas9, dCas9...create lentiviral vectors expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the...wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing other...containing vector using Golden Gate assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for ...assembly of multiple components into a destination vector. The first step in CRISPR/Cas9 Golden Gate multiplexing...to allow for ordered assembly into a destination vector that expresses Cas9. This is illustrated in the...

  9. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through injection...Root DE (2014) Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nat...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ... Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long...

  11. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ... we would recommend using a cDNA overexpression vector rather than going through all the troubleshooting... as WU-CRISPR (Wong et al., 2015)) or our lab’s sgRNA scorer 1.0 (Now 2.0) (Chari et al., 2015) and picking...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...The gRNA oligos are subsequently cloned into your vector of choice using the adapter sequences that you ...28414855. 2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target...Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for CRISPR screening....

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites or gateway compatible...neuronal tools to the expanding gateway compatible vectors currently shared amongst the zebrafish community...discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available at Addgene. ...for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here. Dimidschstein...Very low off-target activity; protein purification vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low ...low off-target activity; mammalian expression vector Rees H, et al. Nature Communications. 2017. PubMed... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...respectively. You should also choose between: Dual vector system: express the transactivator and the tet-...-controlled gene of interest from two separate vectors. Stable cell lines or transgenic animals can be...available for many transactivator or tet-inducible vectors. Tetracycline-inducible Backbones Find backbones...

  15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...depending on perspective – to work with inefficient sgRNAs in my initial direct screening efforts, which were...modular toolkits build single plasmid targeting vectors that encode the gRNA transcript and the repair ....114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection...

  16. CRISPR Plasmids - Tagging

    Type
    Collection
    ...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA, based on ...the isolation of native protein complexes. This vector serves as a backbone to clone the left and right...Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic safe harbor locus...gRNA_AAVS1-T2 (Addgene #41818) or using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_...of HDR, which makes it easy to construct a donor vector, because MMEJ utilizes very short (around 20 bp...

  17. CRISPR Guide

    Type
    Collection
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 - increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription... Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription...pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic promoters. Daniel...119760 pSGDlucV3.0 Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...

  20. CRISPR Guide

    Type
    Guide
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 - increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...
Showing: 41 - 60 of 63 results