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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 46 results for: snap-tag

Showing: 41 - 46 of 46 results

  1. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...and in more than 30 countries around the world. Snapshots of the BioBuilder experience With its intentionally...recognized platform in March to exhibit projects at any stage of completion, from pitch to prototype. Last year...preparing local (Massachusetts), economically disadvantaged students for paid summer internships. A summer...

  2. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...more about the Gateway cloning method and its advantages. An introduction to Gateway technology The Gateway..., etc). This video demonstrates how to use the Snapgene program to design Gateway plasmids.    ...and verify that your construct is functional. Advantages of the Gateway cloning method Compatibility and...JY, Chin CF (2015) Gateway Cloning Technology: Advantages and Drawbacks. Clon Transgen 4:138. doi:10.4172...

  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...possible consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, Subach ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...

  4. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...lineage, without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...reprogramming [1]. The process can be divided into two stages: Dedifferentiation - Conversion of adult somatic...differentiated cells of choice. The dedifferentiation stage involves overexpression of four reprogramming factors...differentiated somatic cell to revert back to a pluripotent stage (iPSC formation) [2, 3]. The iPSCs then proliferate...reprogrammed to iPSCs successfully [10-12]. The next stage of reprogramming consists of redifferentiation of... resources More (due to additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is ...Williams, L.A., B.N. Davis-Dusenbery, and K.C. Eggan, SnapShot: directed differentiation of pluripotent stem ...

  6. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...tendency – mean, median, and mode give you a summary snapshot without telling you anything about outliers or...dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA and MANCOVA...
Showing: 41 - 46 of 46 results