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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,046 results for: ins;

Showing: 291 - 300 of 1046 results

  1. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...Alex Chavez, researcher at the Wyss Institute for Biologically Inspired Engineering and member of the Addgene... vectors (~8.5 kb of insert) and the reduced titer associated with large inserts can make lentivirus production...particular, the Church lab at the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench.... For example, we’ve teamed up with the Broad Institute to provide lentiviruses for delivery of SpCas9...already expressing Cas9. As John Doench (Broad Institute and member of the Addgene Advisory Board) says...Doench/Root labs from the Broad Institute for providing us with advice and expertise as we’ve gotten these...

  2. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...binding with Rep proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important...with the viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate replication... with ITRs that flank the gene cassette, which contains a promoter, the gene of interest, and a polyA ...flip” or “flop” (Figure 2). Additionally, the ITR contains a 4-nucleotide Rep binding element (RBE) that ...resolution site that serves as the target site for Rep proteins (Savvy et al. 2013). Figure 2: AAV2 wild type...type ITR in flip and flop orientation. The ITR contains three palindromic sequences (A-A’, B-B’, and C-C...sequences. Using recombinant-deficient bacterial strains such as NEB Stable, Stbl3, JC8111, SURE2, or XL10...

  3. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...Plasmids expressing fluorescent proteins will be used to tag individual strains of bacteria. Their interactions...diabetes mellitus. Recombinant human proteins that play a role in insulin regulation and diabetes will be ...and the activity of the plant compounds on these proteins will be tested. Investigating changes in neuronal...after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy of...condition is crucial. Plasmids expressing fluorescent proteins and a new two-photon laser scanning microscope...manipulate neural populations in order to get new insights into functional network interactions. Combining...bacterial strain. The sensor will be transported inside a small 3D printed robot that will “swim” in the...

  4. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While...increase the chances of inducing an immune response against the capsids or transgenes. Sensory neurons and ...as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... ...capsids express differently in different mouse strains, a phenomenon that we are calling “strain-specific... but is more effective than AAV9 in many other strains, including FVB/NCrl, 129S1/SvImJ, DBA/2J, and C57BL...table from Huang et al, 2019 for affected mouse strains). However, other capsids cross the BBB via interactions...interactions with LY6C1 are effective in both mouse strains. It is therefore important to review the literature...

  5. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ... a VSV-G envelope expressing plasmid. Envelope proteins determine which types of cells a virus can infect...invariant sgRNA scaffold and cloning sites for inserting the guide sequence (pSpCas9(BB)). The 2A-GFP allows...pCMV-VSV-G was deposited by the Bob Weinberg lab and contains an envelope protein for producing lentiviral and...lentiviral packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used with pMDLg...lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It also requires pRSV-Rev...lab is a 3rd generation lentiviral vector that contains a scrambled shRNA. This scrambled shRNA is used... cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse...

  6. Antibodies 101: Isotypes

    Type
    Blog Post
    .... Antibody heavy chain proteins as well as the genes that encode those proteins are designated by the ...antibody isotypes. Binding to complement system proteins, which can initiate a cascade that helps attack...their own. The antibody’s heavy-chain constant domains, or Fc region, can be bound by special receptors...location of disulfide bonds, the number of constant domains, and the hinge region length. Now for some essential...minor differences in their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2,...IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies. They... any of the other isotype heavy chain constant domains. This switching helps B cells adapt during the ...

  7. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...If you study proteins, you’re probably quite interested in the canonical 20 amino acids. But in your ... add in fluorescent or chemically reactive side chains, all of which can help determine structure and ...for the ncAA integrated into the cell’s genome, instead of incorporation via plasmid or media. Theoretically... systems – the cells growing and producing the proteins – are not, and many of the existing GCE tools ...recombinant expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses the TAG...for the phosphoserine and, because it uses ∆serB strains, does not require exogenously added phosphoserine...checking out all of the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep ...

  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...However, there are instances where traditional base editors are preferred. For instance, if target nucleotides...genome editing technique that mediates targeted insertions, deletions, and all possible base-to-base conversions...pegRNA) that both specifies the target site and contains the desired edit (or edits) engages the prime ...combined with any of the PE2-PE5 strategies. PEmax contains a reverse transcriptase with a sequence optimized...strategies to evolve more effective prime editor proteins, but found that the mutations that emerged depended... PE6a and PE6b are small prime editors with RT domains derived from the E. coli Ec48 retron RT and the... ✓ ✓ ✓ Evolved reverse transcriptase domains Smaller size and/or improved efficiency   ...

  9. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...each mutated allele.  Cre-lox expressing strains Cre-lox strains are a common example of mice that are derived... Crossing mice to generate a desired phenotype Instead, genetic engineering should be used to separately...independently into different mice, and then these strains should be crossed to generate double-mutant offspring...experiments. You should also maintain your parental strains throughout this process. In the F1 and later generations...genetically engineered mice with a single mutation or strains that have been crossed, there are many considerations...gene randomly into their genome, some transgenic strains are difficult to breed. Some problems with transgenic...expression level of the transgene. In existing strains of mice, the transgene can be maintained on a hemizygous...

  10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...and identified interactions of these proteins with human proteins. Their deposited plasmids expressed ...over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman ...infection and how to interfere with it in a BSL-2 lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol...receptor for SARS-CoV. The lab also found that cathepsins play a role in SARS-CoV infection and deposited...Biering et al., 2021). In their search, they found mucins have an important role in viral entry, which are...viral infection.  Expression of SARS-CoV-2 viral proteins In the early weeks of the COVID-19 pandemic, the...expressed 26 of the 29 SARS-CoV-2 proteins which all but one are cloned into pLVX vectors (for lentiviral packaging...
Showing: 291 - 300 of 1046 results