High Complexity Golden Gate Assembly with NEB’s DAD
Type
Blog Post
...rules are: (1) don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same...assemblies are possible, the two sets of data together indicate that 35-pieces is a reasonable upper limit for...Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https...