...acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in...

...to help you learn more about fly biology and fly-based experimental systems. Some resources will direct...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington,...

...find themselves plagued by troubleshooting a virus-based experiment at one point or another. After amplifying...

...the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature information... create their new plasmid.  The Addgene Vector Database has more information about common vector backbones...

...Hopefully we will have new tools for plant science based around those technologies in the near future. Science...

... to determine the concentration of your samples based on your sample’s HC + LC area. Example calculation...

...quality control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible...

...application to neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead...

...desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone...

...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter...

...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...

...could be harnessed or enhanced for future MagLOV-based tools, just as light-induced conformational changes...

...simplicity we will discuss the more common enzyme-based reporter methods. For enzymatic signal detection...

...Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency for...

...appropriately credited, broadening the knowledge base of AAV vector performance in various brain regions...

...Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses...

...followed by sequencing and further data analysis. Based on Figure 31.5.2 from https://pubmed.ncbi.nlm.nih.gov...

...together a really great solution that covered all the bases. You may have also used your writing skills to convey...

...you do? What follows are just a few suggestions based on what I have found to be especially beneficial...

...CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool...