We narrowed to 712 results for: BASE
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TypeBlog Post...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...
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Viral Vectors 101: Voltage Indicators
TypeBlog Post...Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific Reports 9: https... -
Antibodies 101: Flow Compensation
TypeBlog Post...populations. Figure 2: Compensation example based on experimental sample. Flow plots showing a sample... -
Plasmids 101: Degron Tags
TypeBlog Post...in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ... -
Of Myc and Men
TypeBlog Post...Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org ... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...journal clubs have become popular, including those based on Twitter. At my former academic department, we... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in... -
Academic vs. Industry Postdocs
TypeBlog Post...manager: Unlike academia, promotions in industry are based on people’s ability to manage others and work as... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...find themselves plagued by troubleshooting a virus-based experiment at one point or another. After amplifying... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature information... create their new plasmid. The Addgene Vector Database has more information about common vector backbones... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...Hopefully we will have new tools for plant science based around those technologies in the near future. Science... -
Plasmids 101: Codon usage bias
TypeBlog Post...amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. ... at all in E. coli (you can find a variety of databases showing various organisms’ codon preferences online... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...together a really great solution that covered all the bases. You may have also used your writing skills to convey... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...over the other BiFC is a convenient, fluorescence-based method for directly visualizing PPIs in cells. The... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...application to neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead... -
Lab to Office Culture Shock
TypeBlog Post...desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...CREATE method? CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...appropriately credited, broadening the knowledge base of AAV vector performance in various brain regions... -
Bioinformatics at Addgene
TypeBlog Post...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter...