...gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells to target CD19. Immunol...DNA while eliminating some of the drawbacks. Specifically, viruses are able to infect and replicate in...Anderson Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as...

...problems, develop general technical proficiency, communicate your work, maintain ethical standards and interact...? Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... you admire her management style? Does he have ethical behavior? Does the lab encourage diversity? Knowing...

... tactics of prokaryotes to deliver the technological biological blockbuster known as the CRISPR (clustered...destroy the phage upon re-exposure (2, 3). More specifically, specialized Cas proteins snip the foreign DNA...programmable platform well-suited for various innovative biological and translative technologies. Addgene has empowered...

...makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system...from a single construct. DNA and gRNA are not to scale.   Let’s start with the simplest multiplexing...multiplexing plasmid is available from the Koffas lab. Scaling up your multiplexed gRNAs with Golden Gate and ...and Gibson assembly methods If you want to scale up the number of gRNAs in your plasmid, you’ll need to ... combinatorial mammalian genetics.” Journal of Clinical Investigation 125(4) (2015):1603-19. PubMed PMID...

... years of exclusively writing lab reports and empirical essays had also stirred some of my more creative...exhibitions, animation, graphic design, and more. I recall a particularly busy point of the first semester... notion of “heading” in soccer as a possible neurological health risk. Having played the sport my entire...among collision sport athletes, as well as the ethical and health policy implications arising from sport-related...

... defined learning program. Learners cannot (theoretically, at least) fully and freely engage or disengage...Informal education is education that is provided, typically ad hoc, for learners who can choose to engage ...would use “PCR test” to mean “a test that uses a biological sample to look for presence of a virus.” A scientist... scientist underrepresented minorities in your local high school who are also students in AP biology ...

...editing of transformants then typically occurs in T0 plants (from callus) or T1 plants (Arabidopsis floral...involves killing the untransformed seedlings or callus (undifferentiated plant cell culture), which is...cacti and beets. Betanin belongs to a different chemical family than the red/purple plant pigments found...

...bonds. This post focuses on "sticky end" TOPO (also called TOPO-TA) cloning; however, the TOPO cloning technique...to facilitate replication. The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I... has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading functionality are...are planning on transforming your reaction into chemically or electro-competent E. coli - excess salt causes...

...FRET reporters. mTFP1 is also insensitive to physiologically relevant changes in pH. Find mTFP1 plasmids...Fluorescent proteins that oligomerize often affect the localization and/or function of the protein that they are...fluorescent protein and found that the fusion protein localized to their expected locations. An analysis of mWasabi...characterization and applications in fluorescence imaging." Biochemical Journal 400.3 (2006): 531-540. PubMed PMID: 16859491...

... PA will put their award to work on a condition called xeroderma pigmentosum (XP). This recessive genetic...gene encodes a sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they...Wattanasirichaigoon carried different mutations in a gene called SOX10. To understand the functional role of those...models carrying different SOX10 mutations. More specifically, they’ve proposed to use plasmids with modified...

... The conventional approach has typically been to either systematically delete/overexpress endogenous genes... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...

...test if a type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 virus. But...Then, one of them heard about our online platform, called Crowdfight, and filed a request for help. In just... stage of the project we are focusing on what we call “asymmetric collaborations.” These are transient...-and-error. A recommendation for the correct statistical test can save a retraction. Today, collaborations...

...Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene ... with biorender.com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends ...elements of AAV and rAAV vectors, modifications can drastically affect gene transfer efficiency and transgene...serotypes vary in their gene transfer efficiency. Our practical knowledge of ITRs is still limited, but this area...

...Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name 104496  AAV1...fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited PSAM AAV...

...cell death" plays a pivotal role in an array of biological processes including development, the immune system...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters... reporter for imaging apoptosis in vivo." Cell chemical biology 23.7 (2016): 875-882. PubMed PMID: 27447051...apoptosis in animals." Journal of the American Chemical Society (2019). PubMed PMID: 30821975. Additional...

...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without...Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor labeling...variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific loci based on the guide RNA sequence...RFP) to the genomic loci of interest. Pairs of identical hairpins within one gRNA will result in a blue...

...from Massachusetts General Hospital and Harvard Medical School took a closer look at the AAV vectors that...Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving iodixanol gradients...exo-AAVs seem to target cell types with a similar specifically as conventionally produced AAV particles. But... Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development 9 (2018): 278-287. PubMed PMID: 29707602...

...to directly activate and inactivate biological processes. Chemical effectors are suboptimal for a number...neuroscience. In fact, precise light-based control of biological processes is highly useful in other fields, including...system to develop a bacterial camera that prints a chemical image, as well as a genetic method for the computational...

... outcomes. The quality of the work is often dramatically improved when tasks are distributed appropriately...the project completed by an individual who is a local expert in that task (or has the bandwidth to become.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...recommend putting a regular check-in meeting on the calendar even if it is just 15 minutes a week. Clearly...

... Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking the...your department, and online. The Howard Hughes Medical Institute (HHMI) offers some great resources for...Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management for Postdocs and...