We narrowed to 654 results for: CHI;

1. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ...vectors can be made at high titers equal to those achieved with lentivirus but offer an improved safety profile...

2. Hot Plasmids and Viral Preps - May 2021

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   ...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...

3. Anatomy of a Plasmid Page at Addgene

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   ...know the catalog number of the plasmid you are searching for, this is the quickest and easiest way to find...

4. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...dengue for many years. Since more than a thousand children died of malaria today, let's not risk a potential...

5. Tips for Using BLAST to Verify Plasmids

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   ...sequences and may be the most comprehensive for searching. Timesaving Tip #1: If you know the species that...

6. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...have been developed to recruit transcriptional machinery to dCas9 and its RNA directed binding site. These...

7. MXS Chaining

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   ... 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of DNA molecules...

8. Negative Can Be Positive: Open AAV Data with Addgene

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   ...214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard,...

9. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   ...the accessibility of your target epitope(s). To achieve these goals, researchers commonly use one of two...

10. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ..., P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly...

11. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    ...into the host cell’s genome and then use cell machinery for transcription and translation. Here, we will...

12. DNA Purification Without a Kit

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    ...method from the Bishop Lab at the University of Chicago doesn’t use actual milk to purify DNA, but rather...

13. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...technical expertise in a specific field. While searching for jobs and making connections, avoid falling...

14. Lentiviral Vector Uses and Overview

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    Blog Post

    ...Second, Tat is eliminated through the addition of a chimeric 5’ LTR containing a Tat-independent promoter. ...

15. Cpf1: A New Tool for CRISPR Genome Editing

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    ...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...

16. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...Addgenies from wasting hours of valuable time searching for samples and keeps us from discarding tubes...

17. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...at the center of the structure, shielded from quenching by aqueous solvent. This tightly-packed structure...

18. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...supporting novice to expert-level researchers in achieving consistently successful CRISPR experiments. Jessica...

19. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...strain distribution centers in the US, Europe, and China. In addition, the Zebrafish Model Organism Database...

20. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...vectors, such as adeno-associated virus (AAV), from reaching their targets. The AAV9 serotype can breach the...