We narrowed to 650 results for: CHI;

1. Anatomy of a Plasmid Page at Addgene

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   Blog Post

   ...know the catalog number of the plasmid you are searching for, this is the quickest and easiest way to find...

2. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...dengue for many years. Since more than a thousand children died of malaria today, let's not risk a potential...

3. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ...sequences and may be the most comprehensive for searching. Timesaving Tip #1: If you know the species that...

4. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ...technical expertise in a specific field. While searching for jobs and making connections, avoid falling...

5. MXS Chaining

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   ... 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of DNA molecules...

6. Negative Can Be Positive: Open AAV Data with Addgene

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   ...214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard,...

7. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   ...the accessibility of your target epitope(s). To achieve these goals, researchers commonly use one of two...

8. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ..., P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly...

9. Viral Vectors 101: The Retroviral Lifecycle

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   Blog Post

   ...into the host cell’s genome and then use cell machinery for transcription and translation. Here, we will...

10. DNA Purification Without a Kit

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    Blog Post

    ...method from the Bishop Lab at the University of Chicago doesn’t use actual milk to purify DNA, but rather...

11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

    ...have been developed to recruit transcriptional machinery to dCas9 and its RNA directed binding site. These...

12. Lentiviral Vector Uses and Overview

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    Blog Post

    ...Second, Tat is eliminated through the addition of a chimeric 5’ LTR containing a Tat-independent promoter. ...

13. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...

14. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...Addgenies from wasting hours of valuable time searching for samples and keeps us from discarding tubes...

15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...at the center of the structure, shielded from quenching by aqueous solvent. This tightly-packed structure...

16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...strain distribution centers in the US, Europe, and China. In addition, the Zebrafish Model Organism Database...

17. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ...vectors, such as adeno-associated virus (AAV), from reaching their targets. The AAV9 serotype can breach the...

18. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

    ...protein. To demonstrate the specificity that can be achieved with CRISPRa, the Day lab singled out two Bdnf...

19. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ...in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims should have short titles,...

20. Lab to Office Culture Shock

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    Blog Post

    ...tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of camaraderie again...