Addgene

We narrowed to 628 results for: ESP

Showing: 581 - 600 of 628 results

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The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...SpRY, can target NGN PAMs and NRN and NYN PAMs, respectively (Walton et al., 2020). Characterization of Cas9...
Analyzing CRISPR Editing Results with ICE from Synthego

Type

Blog Post

...sample by clicking on the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth...
CRISPR-based Genome Editing Systems For Editing in E. coli

Type

Blog Post

...of pTargetF into the BL21(DE3)/pCas strain. In response, we modified the pCas/pTargetF system and updated...
Save Time with Transient Plant Leaf Transformations

Type

Blog Post

...recognized or leaky in Agrobacterium. This is especially problematic with reporter genes as you might ...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

...neurotrophic factor (BDNF). Bdnf is expressed early in response to neuronal activity (hence, “immediate-early”...
Antibodies 101: Immunoprecipitation

Type

Blog Post

... slightly higher, than those of agarose beads, despite the size difference. Magnetic beads are particularly...
Hot Plasmids - November 2023

Type

Blog Post

...-iPAK4 expressed under promoters like the tet-responsive element (TRE) or the cFos promoter. For example...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

...titer virus is used, it is not always feasible, especially when the lentiviral stock needs to be titrated...
Plasmids 101: Control Plasmids

Type

Blog Post

...may be, proper controls are a basic tenet of responsible scientific inquiry and investigation. References...
Tips for Using FRET in Your Experiments

Type

Blog Post

...stoichiometry of donor:acceptor is difficult to address especially when you investigate unknown molecular complexes...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

...a fluorophore, not every marker will have a corresponding fluorophore-conjugated antibody commercially...
Transferable Skills Guide: Time Management

Type

Blog Post

...used to do mammalian cell culture, I would often respond to friends asking me to dinner saying something...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

...downstream of the promoter in Brainbow 1.0 and 1.1, respectively, cells will fluoresce either red or orange until...
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Type

Blog Post

...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene products were...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

...only saw incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our...
Five Popular Model Organisms

Type

Blog Post

...models of human AML accurately predict chemotherapy response." Genes & development 23.7 (2009): 877-889. PubMed...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

...miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their emission spectra. These...
New Optogenetic Tools for Cytoskeleton and Membrane Control

Type

Blog Post

...activate the Rho GTPases Cdc42, Rac1, and RhoA, respectively) using the iLID system (Mahlandt et al., 2023...
Thoughts on the Future of Research 2015 Boston Symposium

Type

Blog Post

...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...

Showing: 581 - 600 of 628 results

Results per page:

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Analyzing CRISPR Editing Results with ICE from Synthego

CRISPR-based Genome Editing Systems For Editing in E. coli

Save Time with Transient Plant Leaf Transformations

New Tools Enable CRISPRa for Neuroscience Applications

Antibodies 101: Immunoprecipitation

Hot Plasmids - November 2023

Getting the Most from Your Lentiviral Transduction

Plasmids 101: Control Plasmids

Tips for Using FRET in Your Experiments

Antibodies 101: Beyond Surface Labeling

Transferable Skills Guide: Time Management

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Five Popular Model Organisms

Quick Guide to Near-Infrared Fluorescent Proteins

New Optogenetic Tools for Cytoskeleton and Membrane Control

Thoughts on the Future of Research 2015 Boston Symposium

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