We narrowed to 637 results for: ESP

1. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   ...sample by clicking on the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth...

2. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   ...However, the Sendai virus method is expensive, especially if used to make clinical grade hiPSCs. For example...

3. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ...Specialist, Technical Support Advisor, Technical Correspondent or similar. While there isn’t a clear distinction...

4. Hot Plasmids - November 2023

   Type

   Blog Post

   ...-iPAK4 expressed under promoters like the tet-responsive element (TRE) or the cFos promoter. For example...

5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   ...SpRY, can target NGN PAMs and NRN and NYN PAMs, respectively (Walton et al., 2020). Characterization of Cas9...

6. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   ...References  del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication and Control...

7. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   ...chunks, we have the flexibility to adapt and easily respond to changing needs of the teams at Addgene. Another...

8. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   ...recognized or leaky in Agrobacterium. This is especially problematic with reporter genes as you might ...

9. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   ...neurotrophic factor (BDNF). Bdnf is expressed early in response to neuronal activity (hence, “immediate-early”...

10. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    ...questions can derail your talk. You need to balance respectfully answering questions while steering the conversation...

11. Lab to Office Culture Shock

    Type

    Blog Post

    ... to which she was applying. I probably did not respond to her question as well then as I would now. I ...

12. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    ...deaminate cytosine placed in 2-6 bp and 4-8 bp, respectively, of the protospacer. And if the target cytosine...

13. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    ...in sufficient quantities to generate an immune response. As a proof of concept, they tested a lux-mediated...

14. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ...nonfunctional proteins and protein fragments respectively. Solving the problem of codon usage bias - codon...

15. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    ...’ end of  the tRNA binds the amino acid that corresponds to the anticodon sequence. That means there are...

16. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    ...Cells Induces Gamma Rhythm and Controls Sensory Responses.” Nature 459(7247): 663–67. PubMed PMID: 19396156...

17. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    ...titer virus is used, it is not always feasible, especially when the lentiviral stock needs to be titrated...

18. Plasmids 101: Control Plasmids

    Type

    Blog Post

    ...may be, proper controls are a basic tenet of responsible scientific inquiry and investigation. References...

19. Tips for Using FRET in Your Experiments

    Type

    Blog Post

    ...stoichiometry of donor:acceptor is difficult to address especially when you investigate unknown molecular complexes...

20. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    ...a fluorophore, not every marker will have a corresponding fluorophore-conjugated antibody commercially...