We narrowed to 672 results for: FIE

1. Addgene's Cancer Collection Pages Connect the Research Community

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   ...always effective. This is why recent work in the field of cancer research has focused on picking apart ...

2. Plasmids 101: Optimizing Plasmid Yields

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   ...cell in your culture. Copy number is partially specified by a plasmid’s origin of replication, but can ...

3. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...Zacharias, D.A., et al., Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live...

4. Experimenting with New Careers while in Grad School

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   ...Your Research Learn About A Variety of Different Fields on Our Collections Pages  ...

5. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...drives? Then do so! It's a tremendously promising field that could address many pressing global problems...

6. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...

7. Plasmids 101: Multicistronic Vectors

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   ...innovative solutions necessary for advancing the field. References: 1. Internal initiation of translation...

8. Tips for Using BLAST to Verify Plasmids

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   ... your plasmids instead! All BLAST images are modified screen shots from the NCBI BLAST website. Additional...

9. Great Results Start with Great Standard Curves

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   ...should use a different method of analysis. Different fields and assays have varying criteria for an acceptable...

10. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy et al...

11. An Introduction to Adenovirus

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    ...)00023-6. PMID: 12727116. Borkenhagen, L. K., Fieldhouse, J. K., Seto, D., & Gray, G. C. (2019). Are adenoviruses...

12. CRISPR 101: Making a Knock-In Cell Line

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    .... An efficient gene knock-in strategy using 5’-modified dsDNA donors with short homology arms. Nat Chem...

13. MXS Chaining

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    ...functions, and thus, is used in many biological fields. In synthetic biology for example, high-throughput...

14. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...reduced chance for off-target effects. As the CRISPR field continues to evolve, it is likely that the use of...

15. SciComm: Taking Science to Elementary and Middle Schools

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    ...Genome engineering, and many other technologies and fields in biology, are moving so quickly that it’s basically...

16. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...with the role our repository has played in these fields, and seeing names like Feng Zhang, Jennifer Doudna...

17. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...Addgene homepage. You may have noticed the search field front and center. Let’s type in “crispr” and see...

18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield JF, Doudna JA (2020) Targeted Genome Editing of...

19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ... Fluorogen activating proteins (FAPs) – These modified antibody fragments bind and enhance small molecule...

20. Plasmids 101: Colony PCR

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    ...insert-specific primers. If your negative control amplifies a product of the expected size, you know the genome...