We narrowed to 876 results for: NSI

1. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   ... in the human genome. The pair has focused considerable attention on transcription factors, starting... Myers: Only a small percentage of the proteins inside a cell have a commercial antibody against them....

2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   ... transport miRNA from drug-resistant cells to sensitive ones. Though this process is not fully understood...malignancy, concluding that these cells may be considered "natural tumor suppressors." A growing interest...

3. What's New in CRISPR - November 2021

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   ...increase activity and decrease the gRNA size by intensively remodelling the gRNA scaffold through five rounds...tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable of correcting...

4. Seven Tips for Using LinkedIn as a Scientist

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   ...your network. If someone has connected with me, I consider them fair game for connecting to others.  Pro ...including and especially the hiring manager. This is considered intrusive and it’s still connecting with a stranger...

5. Troubleshooting Your Plasmid Cloning Experiment

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   ...Streak on them an E. coli  strain known to be sensitive to that antibiotic, and if the strain is growing...positive clone after these steps, you might want to reconsider your approach, and try a different cloning strategy...

6. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   ...even come [to grad school]!” He asked if she had considered other careers, which she hadn’t.  He then said.... “I was lucky to have been able to make that transition really smoothly,” says Susan. After being in ...

7. Lighting Up Cell Signaling with Photoswitchable Kinases

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   ...phosphorylated MEK1/2 levels, suggesting PP1/PP2A is responsible for this MEK1/2 negative feedback. 3. Studying...Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in...

8. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...be used to measure your fluorescent protein’s propensity to aggregate. The basics and pitfalls of these... the best way to test a fluorescent protein’s propensity to homodimerize. Several studies have used the...

9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during cell division....E. coli do not contain the F plasmid (and are considered F-); however, there are a few popular lab strains...

10. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    ...naturally in camels, consist of a single heavy chain, and they are small and stable inside cells. Nanobodies...

11. New Tool for Lineage Tracing: The ClonTracer Library

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    ...abundance before and after a treatment. The library consists of a pool of lentiviral vectors with roughly 73...populations, they found that similar barcodes consistently made up the majority of the resistant populations...

12. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...Polyclonal antibodies are produced in animals and consist of a mixture of antibodies recognizing many different...hybridomas.  First, the long-term stability, consistency between batches, and molecular definition of ...

13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ... example, the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter...any template DNA and primers.  After performing extensive QC on that sample, we’ll then load it on to our...

14. Corralling and Cornering the Goldilocks Antibody

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    ...scientists to confidently choose reliable and consistent antibodies for specific markers. The Screening...production methods    NeuroMab and others have an extensive library of monoclonal antibodies; Addgene collaborates...

15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ... toolkit from Benjamin Field’s lab. Special Considerations There are a few special exceptions to the usual...constructs or certain cell environments). Also consider that a protein fusion may disrupt functionality...

16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...   Let me leave you with a few practical considerations: Design your gRNA to overlap the CpG of interest...interest. Having the CpG directly inside the gRNA sequence is a great guarantee that DNMT1 will be blocked...

17. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...and mice with Nanoblades CRISPR has also been extensively used to create transgenic mice by microinjecting...post Resources on Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources...

18. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...Special breeding cases Whether your mouse colony consists of genetically engineered mice with a single mutation...strains that have been crossed, there are many considerations for effectively breeding your mice. Because...

19. Engaging with science and society at pgEd

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    ...institutions. The conversations often move quickly into sensitive topics and uncomfortable places, from sticky ethical...rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull...

20. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ... 1000000097) The Winslow lab barcoding library consists of 96 retroviral plasmids (based on the Murine...mammalian cells. The MuLE system kit from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral...