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  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during cell division....E. coli do not contain the F plasmid (and are considered F-); however, there are a few popular lab strains...

  3. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...your network. If someone has connected with me, I consider them fair game for connecting to others.  Pro ...including and especially the hiring manager. This is considered intrusive and it’s still connecting with a stranger...

  4. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Streak on them an E. coli  strain known to be sensitive to that antibiotic, and if the strain is growing...positive clone after these steps, you might want to reconsider your approach, and try a different cloning strategy...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...increase activity and decrease the gRNA size by intensively remodelling the gRNA scaffold through five rounds...tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable of correcting...

  8. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...abundance before and after a treatment. The library consists of a pool of lentiviral vectors with roughly 73...populations, they found that similar barcodes consistently made up the majority of the resistant populations...

  9. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...abundance of your targets isn’t too different, consider antibody affinity: stronger binding antibodies...membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won’t evenly re-absorb...

  10. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...the ‘quality’ of the gRNA sequence, including propensity for off-targets, secondary structures, etc., ...more pan-genome PAM flexibility, you’ll want to consider one of several Cas9s that recognize NG PAMs. The...

  11. Viral Vectors 101: Chemogenetics

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    Blog Post
    ... crossing the blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies...response you aim to manipulate. It is important to consider that the same receptor can elicit different responses...

  12. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...scientists to confidently choose reliable and consistent antibodies for specific markers. The Screening...production methods    NeuroMab and others have an extensive library of monoclonal antibodies; Addgene collaborates...

  17. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...Special breeding cases Whether your mouse colony consists of genetically engineered mice with a single mutation...strains that have been crossed, there are many considerations for effectively breeding your mice. Because...
Showing: 581 - 600 of 873 results