Light Sheet Fluorescence Microscopy
Type
Blog Post
...of LSFM microscopes (3,4).
After this initial application, Stelzer’s group described the single-plane or...
We narrowed to 653 results for: PPL
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Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post...plasmid with a downloadable protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by... -
Which Fluorescent Protein Should I Use?
TypeBlog Post.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues” Physiol... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall... -
AAVs in Retinal Gene Therapy
TypeBlog Post...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post..., Alexa Fluor 568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores are often bulky and... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...or growth stage). A prey library can either be applied to the bait in a single population (e.g. transforming... -
Plasmids 101: Protein tags
TypeBlog Post...deciding on which one to use will depend on your application (see table below). The antibodies available for... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post...when discussing raising children, it can also be applied to research. Sharing knowledge and tools is the... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post...Modular Cloning Plasmids 101: Modular Cloning Applications and Kits Plasmids 101: Golden Gate Cloning ... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides the missing... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post...researchers can now select the best tool for their application from among many options. Conclusion With these... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...viral vector service in 2016. We realized we could apply the lessons learned from our plasmid lab to develop... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016...