We narrowed to 651 results for: PPL

1. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...lies in the diversity of nucleases and their applications. It will be interesting to see what new editing...

2. Light Sheet Fluorescence Microscopy

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   ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...

3. Which Fluorescent Protein Should I Use?

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   .... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300...

5. CRISPR 101: RNA Editing with Cas13

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   ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

6. AAVs in Retinal Gene Therapy

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   ...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

7. Plasmids 101: Secondary Nanobody Toolbox

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   ..., Alexa Fluor 568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color...

8. Viral Vectors 101: The Retroviral Lifecycle

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   ...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral...

9. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall...

10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ...labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores are often bulky and...

11. Tips for Screening with Yeast Two Hybrid Systems

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    ...or growth stage). A prey library can either be applied to the bait in a single population (e.g. transforming...

12. Plasmids 101: Protein tags

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    ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

13. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...plasmid with a downloadable protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by...

14. To Codon Optimize or Not: That is the Question

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    ...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org...

15. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR protein adapted for...

16. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...noise, of dsDNA breaks caused by CRISPR. See supplemental figure 6 for more details. Safe-targeting guides...

17. Save Time with Transient Plant Leaf Transformations

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    ...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration...

18. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ...

19. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...viral vector service in 2016. We realized we could apply the lessons learned from our plasmid lab to develop...

20. Plasmids 101: Shuttle Vectors

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    ...vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides the missing...