We narrowed to 783 results for: SCR;

1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   Blog Post

   ...Center are now available at Addgene along with a description of their experimental protocol. The three plasmids...

2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   ...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...

3. Hot Plasmids: Summer 2025

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   ... separately). Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE ...

4. Twenty Years of Addgene Sharing: CRISPR

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   ...double-stranded break, while modulators regulate transcription and chimeric efforts make a precise edit or ...

5. Plasmids 101: Degron Tags

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   ...proteasome for degradation. Like several other tags described here, the dTAG uses a small molecule, a tag, and...

6. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   ...signature upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical...

7. Of Myc and Men

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   ...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...

8. PhD Applications After COVID

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   ...lab, research, and provide them with a CV or transcripts if requested Thank them, and eagerly await a ...

9. Plasmids 101: Optimizing Plasmid Yields

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   ...concentration Low copy plasmids produce fewer transcripts of their antibiotic resistance genes and can ...

10. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    ...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in...

11. Tips for arabidopsis transformation

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    Blog Post

    ...generate transgenic Arabidopsis, from studying transcriptional and translational dynamics of genes and proteins...

12. Simplify Cloning with in vivo Assembly

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    ... minimize false positive colonies. We routinely screen just two colonies for simple cloning procedures...

13. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...served. I hope that with some of the information described above you feel empowered to start your own journal...

14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR/sequencing ...

15. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post

    ... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...

16. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...Antibiotic Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

17. An Introduction to Adenovirus

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    ... (and therefore between early and late gene transcription) is demarcated by viral genome replication (...

18. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ...reading up on validating genome edits to find screening strategies for your experiment. Also learn about...

19. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ...side of things from taking too much of your time, scroll down for some tips on how to make a successful ...

20. Plasmids 101: Protein tags

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    Blog Post

    ...examples. The tables list more common tags than are described in the post, but have been categorized to help...