We narrowed to 609 results for: SHE

1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   ...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

2. Harnessing Bacterial Toxins for Allelic Exchange

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   Blog Post

   ... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...

3. Five Popular Model Organisms, Part 2

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   Blog Post

   ...with their regenerative abilities is expected to shed light on human aging pathological developments (...

4. Fluorescent Proteins 101: When GFP lets you down

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   Blog Post

   ...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

5. CRISPR 101: Homology Directed Repair

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   Blog Post

   ...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

6. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   Blog Post

   ...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...

7. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   Blog Post

   ... GeneArt® Seamless Cloning and Assembly (Thermo Fisher Scientific) NEBuilder® HiFi DNA Assembly (NEB) ...

8. Virus Protocol - Generating Stable Cell Lines

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   Protocol

   ...deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted to alternative...

9. Plasmid Cloning by PCR (with Protocols)

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   Protocol

   ...amount of recipient plasmid to insert. Isolate the Finished Plasmid Finally, you will need to pick individual...

10. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post

    ...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

11. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

12. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    ...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

13. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post

    ...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...

14. Plasmids 101: Restriction Cloning

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    Blog Post

    ...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

15. AAV ddPCR Titration

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    Protocol

    ...dUTP, Bio-Rad,1863023 10% Poloxamer 188, Thermo Fisher, 24040032 Droplet generation oil, Bio-Rad, 1863005...

16. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

17. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post

    ...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

18. Quick Guide to All Things Lentivirus

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    Blog Post

    ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

19. Typing CRISPR Systems

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    Blog Post

    ... Alyssa Shepard...

20. Antibodies 101: Introduction to Antibodies

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    Blog Post

    ...out of the solution. Once the unbound protein is washed away, the protein of interest is dissociated from...