We narrowed to 665 results for: SIM

...difficulty answering questions. Or perhaps you’re simply attending, but uncertain how to capitalize on your...

...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...

... of different pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to...

...looking at protocols used for libraries that are similar in size and composition as examples. Table 1 provides...

... and requesting each year (Figure 2). We did a similar analysis with CRISPR pooled libraries, although...

...inexpensive and can be 5’-phosphorylated in house using a simple PNK reaction or can be purchased 5’-modified from...

...yields. Why would volume make a difference? In the simplest of terms, more volume = more cells = more plasmid...

...researchers. It is an excellent way to meet people with similar interests! Especially as someone interested in ...

...depositing lab. There may be other plasmids with similar names in the collection, but you can use this name...

...transparent organisms. Advances in tissue clearing Similar to light microscopy, there have been significant...

...organisms with Cas9 risk creating a gene drive? The simplest way to make a gene drive is to insert an endonuclease...

...synthesis as their mode of action and have a very similar relationship as amp and carb (they are interchangeable...

...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ...

...mentioned above, organic solvents can be used to simultaneously fix and permeabilize a sample. But you can ...

...or expertise from the start, or if you can begin simply and build up to more complexity if needed. And ...

...between Jan. 1 and Mar. 1, 2020. Figure 2: A simple approximation of the effect of COVID-19 on data...

...measure the concentration of multiple targets simultaneously. For AAV titration, you should only need to...

...and RNA sequencing (Fujita et al., 2015). Using similar affinity purification with an anti-Cas9 antibody...

...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression...