We narrowed to 716 results for: cas

1. Identifying Sequence Elements with SnapGene's Feature Database

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   Blog Post

   ... crafted a detection algorithm that tolerates occasional mismatches or indels. Empirical tests indicated...

2. The Strength of Story Telling

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   Blog Post

   ...your structure. Develop your protagonist In most cases, the protagonist will be you. Make sure to highlight...

3. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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   ...single colonies and testing multiple preps. In some cases, we suggest gel extraction to isolate the full, ...

4. Meet the New Addgene Board Members!

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   ...Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of ...

5. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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   ...mRNA, directly translated into proteins, or, in the case of retroviruses, reverse transcribe their gRNA into...

6. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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   ...transgene. When multiple copies of the transgene cassette are introduced, stochasticity will result in a...

7. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   Blog Post

   ...others, such as AAV2, low yields are the norm. In the case of AAV2 this is thought to be due to the viral particle...

8. Science Careers: Unruly Interests Feed Many Paths

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   Blog Post

   ...’s scientific discoveries into an entertaining podcast? Look at our multimedia producer and online editor...

9. Plasmids 101: TOPO Cloning

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   ...topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening...

10. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    Blog Post

    ...to integrate into the E. coli chromosome in some cases. This terminology held until the 1960s when scientists...

11. Advice for Moving Into Sales After Your Science Postdoc

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    ...apply themselves immediately to the position. In case you are interested yourself, sales and marketing...

12. Mentoring for Scientists: I Have a Mentor, What Now?

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    ...after that will lead to learning. Listen to our podcast interview with Harvard Medical School researcher...

13. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    Blog Post

    ...on the next step of your experiment. But in many cases, you won’t see any signs of DNA in your final tube...

14. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    Blog Post

    ...workshop. “We made sure that we had a backup plan in case someone’s internet cut out. “ On networking Networking...

15. FPbase: A new community-editable fluorescent protein database

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    Blog Post

    ...after submission. Using FPbase The most obvious use case for FPbase is finding information on a particular...

16. Summer SciComm Series: A PhD in Science Communication

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    .... doi:10.1038/nclimate1547 Kunda, Z. (1990). The case for motivated reasoning. Psychological bulletin,...

17. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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    ...collaboration between two research groups. In other cases, the collaborations were simpler and the volunteers...

18. 25 Work From Home Tips for Scientists

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    Blog Post

    ...something like puzzling or crafts, while listening to podcasts made for kids. Plan out meals and snacks ahead...

19. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

    ...Recombinant AAV vector with ITRs that flank the gene cassette, which contains a promoter, the gene of interest...

20. Visualizing Translation at the Single Molecule Level

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    Blog Post

    ...dynamics, but they come with a few caveats. In both cases, creating a reporter mRNA requires inserting multiple...