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We narrowed to 594 results for: cel.2

Showing: 581 - 594 of 594 results

  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....experiments possible in a wide range of organisms and cell lines. In C. elegans, one of the model organisms... efficiency. Finally, as shown in Drosophila S2 cells (Böttcher et al., 2014), inactivation of non-homologous... community. Developments in mammalian and yeast cells using modified Cas9 proteins to regulate gene expression...homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org...

  2. Antibody Guide

    Type
    Guide
    ...surface of most immature B cells; function is unknown Monomer with a valency of 2 IgE Protects against parasites...such as anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found...secondary immune response Monomer with a valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary... ranging from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include...used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared to alternative... produced. Cells - Antibodies can also be produced in cell cultures using hybridomas, cell cultures created...created by fusing B cells with myeloma cells (immortalized B cells). The genetic reorganization that creates...

  3. Finding Your Perfect Job After University

    Type
    Blog Post
    ...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in...that, when those contracts fall through or are cancelled, then redundancies can be made within the company...

  4. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...ask new and interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the ... optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance...

  5. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure...ligase bound to nicked DNA-adenylate." Molecular cell26.2 (2007): 257-271. PubMed PMID: 17466627. 25. Luo...electrophoresis as a sensitive, high-throughput method to accelerate characterization of nucleic acid metabolic enzymes...

  6. Promoters

    Type
    Guide
    ...positions from the transcription start site (TSS) (Figure 2). The Pribnow box (TATAAT) is located at the -10 position...recognizes different core promoter sequences. Figure 2. Basic structure of a bacterial promoter. TSS = transcription...element controls the rate of transcription. Bacterial cells contain sigma factors that assist the RNA polymerase...other promoters can drive expression in specific cells or under different conditions. Promoter Expression...ribosomal RNA (rRNA), which is a main component of a cell’s ribosome structure. Ribosomes are the site of protein...

  7. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ... overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Notes...bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria... and you will not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can...

  8. Science Guides

    Type
    Guide
    ...specific control of activity. Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic...receptors to selectively control and investigate cell signaling pathways. Receptors are remotely controlled...and genetic engineering to measure and manipulate cells (frequently neurons) and their governing biomolecular...detect, measure, and control molecular signals and cells in order to understand their functions. Read More...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...range of infectivity for different cell types (known as tropism). Figure 2: Gamma-retroviral plasmids. Created...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or...-activated cell sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded...actively dividing cell types, whereas gamma-retroviruses can only infect mitotically active cell types. This...Using Packaging Cell Lines Gamma-retroviral vectors can be packaged using helper-free cell lines (Figure...-retroviral packaging cell line developed by Garry Nolan at Stanford. These cell lines contain gag-pol...and rat cells), or an amphotropic envelope, Phoenix-AMPHO (for the infection of mammalian cells). Using...

  10. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...10μL reaction for 5X buffer) 0.5-1μL T4 DNA Ligase H 2 O to a total of 10μL Notes: If the DNA concentrations...complete plasmid can be transformed into bacterial cells for propagation. The majority of ligation reactions...Interpretation Uncut vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid...

  11. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...DNA concentration alone. One method is to conduct 2 ligations for each plasmid you are trying to create...use electro-competent cells instead of the more common chemically-competent cells. The number of bacterial...might need to express YGOI in cultured mammalian cells. The problem is that the only version of full-length... manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-...1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total...colonies, you might want to use higher competency cells. Additionally, if your final product is going to...

  12. Molecular Biology Reference

    Type
    Guide
    ...unique restriction enzyme recognition site (Figure 2). These elements allow for the propagation of the ...elements are summarized in the table below. Figure 2: General structure of a plasmid. Plasmid Element Description... plasmid DNA is replicated upon cell division, and each daughter cell receives at least one copy of the...segregated to the resulting daughter cells. These daughter cells are called clones, since they contain...non-coding RNA (ncRNA) and play important roles in cellular processes and gene regulation. Regions that code...techniques enable the isolation and purification of cellular components, such as DNA, but in practice this ..., which take up plasmids and amplify them during cell division. Because bacteria are easy to grow, divide...

  13. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...DNA concentration alone. One method is to conduct 2 ligations for each plasmid you are trying to create...use electro-competent cells instead of the more common chemically-competent cells. The number of bacterial... manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-...1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total...colonies, you might want to use higher competency cells. Additionally, if your final product is going to...

  14. Molecular Cloning Techniques

    Type
    Guide
    ...Read more in our Gateway Cloning blog post . Figure 2: Summary of Gateway cloning. Created with BioRender.com...homology. While this approach doesn't work in other cell types, it can be a major time- and cost-saver for...
Showing: 581 - 594 of 594 results