We narrowed to 876 results for: lic

1. Choosing the B(right)est Fluorescent Protein: Photostability

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   ...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...

2. Troubleshooting Your Plasmid Cloning Experiment

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   ... this stage and that can be used to simulate in silico cloning. This will definitely help in understanding...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

3. Plasmid Tools for Microbiome Studies

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   ...modular suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow ...Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad host-range...

4. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   ...were at institutions where no data had been made publicly available (below). However, on November 22nd a...compelled at the state level, in the case of some public institutions) to cancel plans to raise salaries...

5. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...   With the addition of blue light, the terminal helices of the LOV2 domain unfold, disrupting the protein...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...

6. Antibodies 101: Conjugation

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   ...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...

7. All in a Twist: dsRNA

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   ...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...

8. Antibodies 101: Secondary Antibodies

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   ...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ...

9. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   ... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...

10. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    ...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...

11. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...Each half of the base editor is fused to a fast-splicing split intein and co-infected into the cell. Intein...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...

12. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ..., excellent resources with effective gRNAs are publicly available, including those found at Addgene! ...realization of a variety of perturbations and applications in functional genomics, cell reprogramming, ...

13. Plasmids 101: Golden Gate Cloning

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    ...and exciting applications, this system is an incredibly powerful tool for cloning complicated constructs...

14. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...looking for a particular protocol or are trying to replicate an experiment, the methods section probably won...is science’s way of validating work prior to publication, it doesn’t mean that there are no mistakes or...

15. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ... perspective and you can layer them for more complicated functions. Those things are easy with CRISPR....just using what biology has given us. Wood is a complicated material and we can access it, but we are stuck...

16. Plasmids 101: Cre-lox

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    ...important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance markers...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...

17. 5 Tips for Troubleshooting Viral Transductions

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    ...experiment and they don't do anything. Viruses are complicated and they perform complex biological tasks, both...vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying...

18. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ... which means they are non-infectious and non-replicative. Nanoblades are produced by transfecting the ...

19. Hot Plasmids and Viral Preps - January 2021

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    ...BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

20. Hot Plasmids - October 2020

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    ...Jerome Goudeau, Cynthia Kenyon, and colleagues at Calico Life Sciences. Like many split FPs, split-wrmScarlet...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...