We narrowed to 862 results for: lic

1. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...

2. Antibodies 101: ChIP

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   ...next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions, and can give... upfront investment than many other antibody applications, this investment can well be worth it once you...

3. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...light-mediated suppression of synaptic transmission in brain slices from mouse, and showed that it can alter motor ...

4. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ...

5. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   ...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...which is both small and potentially less likely to elicit a strong immune response, factors that may make...

6. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   ...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...

7. Top Requested Lentivirus and AAV of 2016

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   ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ... PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers...

8. Antibodies 101: Fab Fragments

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   ... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...fragments described above can be used for similar applications and have similar advantages, though with some...

9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   ...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...Each half of the base editor is fused to a fast-splicing split intein and co-infected into the cell. Intein...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...

11. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...   With the addition of blue light, the terminal helices of the LOV2 domain unfold, disrupting the protein...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...

12. Antibodies 101: Secondary Antibodies

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    ...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ...

13. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    ... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...

14. Troubleshooting Your Plasmid Cloning Experiment

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    ... this stage and that can be used to simulate in silico cloning. This will definitely help in understanding...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

15. Plasmid Tools for Microbiome Studies

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    ...modular suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow ...Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad host-range...

16. All in a Twist: dsRNA

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    ...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...

17. Antibodies 101: Conjugation

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    ...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...

18. Choosing the B(right)est Fluorescent Protein: Photostability

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    ...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...

19. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    ...were at institutions where no data had been made publicly available (below). However, on November 22nd a...compelled at the state level, in the case of some public institutions) to cancel plans to raise salaries...

20. Developing Lab Management Software for Biology

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    ...can fail but, in our current setup, there are no explicit instructions on what to do when something fails... We can set ourselves up for success by giving explicit instructions to lab members through the software...