What the HEK?
Type
Blog Post
...gene expression, growth rates, etc.). Thus, it’s important to maintain short culture periods (less than 20...
We narrowed to 760 results for: mpo
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Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...neutralize the virus,” says Balazs, emphasizing the importance of the second vaccine dose in mounting a robust... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...transcription.However, this system lacks spatio-temporal regulation, and is difficult to reverse, making... -
Meet the New Addgene Board Members!
TypeBlog Post...wealth of resources on Addgene’s website and blog important. “The experience from the scientific community... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise in vivo measurements... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog Post...makes Keima useful for monitoring mitophagy. It’s important to remember that because Keima’s fluorescent relies... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...test this approach with different capsids. Most importantly, because our AAV yields are improved we will ... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise, the NIST Genome... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...binding kinetics of the target ligand. Another important consideration is whether the affinity of an experimental... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...with every possible option, we place the most important controls front and center, and make specialized... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...stylistic consistency. If you’ve ever doubted the importance of copy editors, the New Yorker’s “Comma Queen... -
Summer SciComm: Science Storytelling
TypeBlog Post...storytelling devices, we sometimes forget the most important thing about science: It’s cool. Like bowties! ... -
Summer SciComm Series: Master of SciComm
TypeBlog Post... to change. Most researchers are aware of the importance of science communication, although the detritus... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post...mentors don’t need to be more senior or more “important”, they just need to know you well enough to ask... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...protein’s entire life-cycle. This makes it nearly impossible for scientists to use GFP to image nascent proteins... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post...boilerplate drudgery to some scientists, MTAs are important—they guarantee certain rights and protections ... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...NgAgo In eukaryotes, Argonaute proteins play an important role in RNA interference, binding RNA guides to... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...certainly noticed: This year, the BeHEARD (Helping Empower and Accelerate Research Discoveries) Award attracted...