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We narrowed to 985 results for: nin

Showing: 581 - 600 of 985 results

  1. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...display, you can start thinking about your next cloning experiment early on. Beyond these simple but powerful...

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...are commonly packaged in 400-500 bp cassettes containing the RNA pol III promoter, gRNA and pol III terminator...gRNA multiplexing, Xie et al. constructed PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice... gRNA constructs with PTGs. In PTG constructs containing two gRNAs, they found 100% indel frequency at...

  3. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...school and turn it into a biotech company. Transitioning an idea from academia to a startup Mike Koeris...competing in business plan competitions. After much learning, the focus of the application was changed, but...million. The biggest challenge during this time was learning to communicate effectively to non-scientific audiences...

  4. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ... “Learning is not attained by chance, it must be sought for with ardor and attended to with diligence...lookout for friends or colleagues that have the training or skills you want to learn. Mentors can be senior...asking them to have coffee. This is much less threatening! If they say no, don’t worry about it. It is ...

  5. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that allow you to build ...sequence in the genome in rapid fashion. The two remaining plasmids in the kit have the rest of the protein...takes two to three days from the moment you are designing a TALEN on paper to a finished plasmid. With our...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ... important to know all your options when planning and running experiments. You never know what problems...in an ~50kD protein. Minibodies are bispecific, meaning that each scFv can target a different antigen; ...

  7. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... power (Shaner et al, 2008). In confocal laser scanning microscopy, fluorescent proteins are excited with...photon-excitation, selective plane illumination, TIRF or spinning disk confocal use entirely different excitation...rates can be summarized as follows: Instead of determining a t½ of a fluorescent protein in a water/oil ...

  8. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...particularly interested in whether institutions planning to raise salaries prior to the injunction are ...injunction (see below). Of the institutions who were planning to raise salaries before the injunction, we have... contacted by a number of postdocs who are now planning unionization at their institutions. One clear ...

  9. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...this pivotal moment that Teytelman realized that running interactive workshops to train researchers on tools...expanding this team, leading monthly calls, and planning next steps. April Clyburne-Sherin had already ...there, allowing others to have the resources for running their own workshops: April Clyburne-Sherin’s and...

  10. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...management software is not a requisite for a functioning lab, but it is for a scaleable lab. When you ...mirroring the lab procedures, not artificially constraining them). In the second example we have a separate...-and-true tools means less time getting up and running and more time working on interesting biotechnology...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...and many loxP-containing alleles have also been constructed. Additionally, Cre-containing adenovirus (Ad-Cre...only in specified cells or at specified times. Combining this with some of the loxP methods described above...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    ...fluorescence from other regions of the cell. By combining vectors expressing different fluorescent proteins...can be used to determine cell cycle length by examining the ratio of blue to red cells. Cells undergoing...Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express...

  14. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... working in the pharmaceuticals industry after earning my Master’s in 2016 and then working four more ...to get enough sleep the night before. Have your morning coffee, if that’s part of your daily routine. Stay...s Science Career Guide Check out Addgene’s job openings  ...

  15. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...expression in the retina.  Four approaches for designing synthetic promoters  To build a library of synthetic...marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. Performance ...expression occurred in one type of cell, with the remaining expression occurring in only Müller glia cells...

  17. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...underlying genetic causes of some human conditions, opening up new avenues for therapeutics that treat disease...immunological reconstitution with a normally functioning T cell repertoire (1). In this example, it was...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

  18. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd) photodimers... protein to date By Justin Ng High-throughput screening methods, such as fluorescence-activated cell sorting...s lab developed a new form of FACS-based cell screening, Single-cell Phenotypic Observation and Tagging...

  19. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...are used as it eliminates the need for antibody staining. Check Out Our In-Depth Fluoresence Titering Protocol...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

  20. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...content. Here are some of my tips to organizing and running a memorable panel discussion. Check out Joanne'...where the questions are obvious, the answers uninteresting, the panel members all say the same thing and...will thank you. Own it. For more great tips on running panel discussions see Guidelines for Great Panels...
Showing: 581 - 600 of 985 results