We narrowed to 734 results for: son
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TypeBlog Post...mismatches or indels. Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity...
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Viral Vectors 101: Transductions
TypeBlog Post... will not stably integrate (unless you used a transposon system, CRISPR targeted strategy, etc.) and episomal... -
Antibodies 101: Epitope Tags
TypeBlog Post...they exist but are unreliable for a variety of reasons (Baker, Nature). Tagging your protein allows you... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post... Morelock, M. M., Rothlein, R., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R.,... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post... light. Inspired by @UriAlonWeizmann, here my song about our paper recently published in @nchembio ... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog Post...introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the limited expression... -
Plasmids 101: TOPO Cloning
TypeBlog Post... Lianna Swanson... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...shift won’t happen overnight, but there are a few reasons why you might choose NgAgo over CRISPR proteins... -
Plasmid Cloning by PCR
TypeBlog Post...to add overhangs to your insert of interest for Gibson assembly. The steps following primer design and... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...functionality of the RNA scaffolds follows the rules of Watson-Crick base pairing, allowing for rational, straightforward... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other conditions.... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...be more difficult in the virtual format than in-person. You might miss out on the short chats between ... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post... neurons near the target site.” pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...reporters have been developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...26808669. PubMed Central PMCID: PMC4916159. 2. Jacobson, K., Rajfur, Z., Vitriol, E., & Hahn, K. (2008... -
The MTA at Addgene
TypeBlog Post... Rachel Leeson... -
Hot Plasmids - January 2023
TypeBlog Post...reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies may just not perform as... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...takes time away from our research. For all these reasons, the QCRG is delighted to be partnering with Addgene...