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We narrowed to 1,011 results for: CAL;

Showing: 601 - 620 of 1011 results

  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene ... with biorender.com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends ...elements of AAV and rAAV vectors, modifications can drastically affect gene transfer efficiency and transgene...serotypes vary in their gene transfer efficiency. Our practical knowledge of ITRs is still limited, but this area...

  2. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...to directly activate and inactivate biological processes. Chemical effectors are suboptimal for a number...neuroscience. In fact, precise light-based control of biological processes is highly useful in other fields, including...system to develop a bacterial camera that prints a chemical image, as well as a genetic method for the computational...

  3. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... outcomes. The quality of the work is often dramatically improved when tasks are distributed appropriately...the project completed by an individual who is a local expert in that task (or has the bandwidth to become.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...recommend putting a regular check-in meeting on the calendar even if it is just 15 minutes a week. Clearly...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking the...your department, and online. The Howard Hughes Medical Institute (HHMI) offers some great resources for...Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management for Postdocs and...

  5. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...to think about DNA in this context, but also specifically study it. They might be researching enhancer...--directly or indirectly--many other areas of biological research. If you’re looking for resources that...you study how chromatin effects your favorite biological phenomenon, there’s a reasonable good chance ...RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl ...

  6. Antibodies 101: ChIP

    Type
    Blog Post
    ...your sample before moving on to the next step. Typically, this can be done by cutting the tissue sample...into random, small pieces of roughly equal size, typically between 200-1000 bp. This is to ensure that your...2) fragmented the DNA (3) used antibodies to specifically select for proteins of interest (4) isolated... be a good idea to add "patience and an extra-methodical approach" to the top of your protocols when embarking...

  7. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...1:1 ratio and then cultured in a special medium called HAT. This medium selects against unfused myeloma...antibody, meaning that the antibodies are largely identical between lots.  Disadvantages of monoclonal antibodies...there will be changes between lots over time. Specifically, the antigen-binding sites of the antibody -...2016) Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007. https://doi.org/10.1074...

  8. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...upon infecting a host cell. This viral DNA (also called proviral DNA) is then inserted into the host's ...human gene therapy and is currently being used in clinical settings. AAV is also a cool tool in the laboratory...that we’ve reviewed the common viruses used in biological research, you might have an idea which ones could...Introduction to AAV Viral Vectors 101: Viruses as Biological Tools ...

  9. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...Following up its 2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in ...bacteria. These bacteria inhabit cold temperature locales and psychrophilic enzymes help them survive under...forced to express in mesophilic host systems. The so-called “regular” temperatures of growth, say around 37... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...

  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...Multiplexing is key due to the natural redundancy of biological pathways;  to observe a phenotype, the modification.... Though the plasmids made by Xie et. al are specifically designed for plant expression, PTGs will likely...likely be adaptable to many different biological systems. Although Xie et. al characterized the system ... PTGs will likely work well in many different biological systems. Size is also a factor; the pol lll promoter...

  11. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ... Ventures – a local Cambridge biotech venture capital firm with deep ties into the local academic community... As a biomedical engineer and scientist, I like to envision that my research will have a direct impact...communication styles in academia and industry vary drastically, he reassures that one can learn it by reading...

  12. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    .... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...mentoring programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet...present formally often enough) and give honest, critical feedback for improvement. Go to a science or career...their career paths and get their advice. Attend a local networking event together and help each other practice...

  13. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    .... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... work product in action) Can help you learn the local “ropes” and become an insider faster A mentor that...chapters. Find out what other groups have going on locally. Your university probably has an alumni directory...As a future alum, these are people you can “cold call” and ask for some of their time. There are many ...

  14. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ... helped by expanding this team, leading monthly calls, and planning next steps. April Clyburne-Sherin ...first iteration of these workshops took place at Caltech in Jan 2018 and involved presentations and a handout...Not everyone in the group was able to make it physically to instruct the workshop at the conference, but...have the materials and resources to run their own local workshops was a major goal for the team last year...

  15. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    ...because her skill set wasn’t matching up with the local job postings. “There were jobs that I didn’t seem...team meetings and Susan is assigned to cover the Clinical Research Division at Fred Hutch, so she attends...work include: “Listening. Evaluating evidence. Critical thinking. Being able to learn things quickly. ...like she did for the Northwest Association of Biomedical Research. She does, however, recommend using ...

  16. RNA Extraction Without A Kit

    Type
    Blog Post
    ...widely known of these products is TRIzol® (also called TRI Reagent®, RNAzol®, QIAzol®  and sold by many... within cells or tissues- they don’t actually chemically stabilize the RNA molecules (Allewell and Sarma... MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched...Guanidinium Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016...

  17. Summer SciComm: Preprints

    Type
    Blog Post
    ...arXiv.org), but have been only adopted by biological and biomedical scientists (see: bioRxiv.org or medRxiv.org...Preprints can be read freely by anyone and are typically free of charge for authors to post. They can be... be assessed and commented on by readers, typically peers in the field, and updated by the authors over...

  18. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...exciting new project aimed at understanding how a critical cellular pathway is regulated. You’ve read all...do you know about the vendor and the vendor’s technical support? An antibody from a vendor that you use...frequently, with a responsive and knowledgeable technical support team, is a safer bet than one from a large... all validation is equal and you should think critically about what data is and is not shown on an antibody...

  19. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...and can be specifically targeted to a number of different cell types even when systemically administered...with maximum “trunk” space for genetic cargo. Typically, when researchers speak about AAVs, they are actually...ITRs and serotypes The names of AAVs contain two numerical designations, separated by a slash, that indicate...

  20. To Each HIS Own

    Type
    Blog Post
    ...Much of today's biological research requires a close examination of specific proteins within a system...without eluting the target protein and needs to be empirically determined. In addition, some metal ion substrates...function. B, The string of histidines in the tag is typically between 6 to 9 residues long. The small size of...Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification of human insulin-like growth...
Showing: 601 - 620 of 1011 results