We narrowed to 928 results for: Por

...these reagents in both plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free...

...expressing Cas9 and single guided RNA." Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central...

...bromodeoxyuridine (BrdU) can be used. These dyes are incorporated into the DNA during DNA synthesis and then diluted...

...primers for PCR amplification of fragments to incorporate such regions of homology. This video gives a ...

...while less protein leads to a thinner band. It is important to note, however, that you must include a loading...

...binding  Primary antibody specificity is further supported by a pre-immune serum or isotype-specific control...

...to probing with cells in order to optimize this portion of the assay before attempting it with a random...

...commercially available (see below). Conclusion It is important to note that thermostable, high fidelity, nick-selective...

...most effective and accurate way possible it’s important to select the right test for the job (and, uh,...

...means improving research. More specific antibodies support common research tools like western blots, ELISAs...

...target protein: instead, it recognizes a small portion of the protein, known as an epitope. Epitopes are...

...Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime Editing CRISPR 101: Cas9 Structural...

...be entirely verified by sequencing. Upload any support files that will make it easier for scientists to...

... different stages of molecular cloning, it is important to get a quick and accurate reading of DNA concentration...

...toward one another. The size of the primer is very important as well. Short primers are mainly used for amplifying...

...the safest way possible. Protecting yourself is important! Disclaimer: The SOP presented here has been designed...

...initials. Labeling within a laboratory setting is important for organization, and it is recommended that you...

...Conclusion Appropriately weighing reagents is important to ensure the accurate composition of your solutions...

...provided by your polymerase manufacturer. It is very important to remove all free nucleotides from your PCR product...

... office, discusses her career in science, the importance of international collaboration, and offers career...

...primers Watch the Video! Sequence Analysis Verify important plasmid features using sequence analysis RNA Extraction...

...CI) Heat-inactivated FBS Polybrene (10mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium...

...plasmid. However, a liquid culture is capable of supporting a higher density of bacteria and is used to grow...

...Protecting yourself and working smart in the lab is important! Disclaimer: The SOP presented here has been designed...

...transfection efficiencies as measured by the highest proportion of GFP positive cells without limiting cell growth...

...CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium...

...ingredient specifically do? Template DNA: Contains the portion of DNA that we are interested in amplifying for...

...gene of interest Triton X-100 Benzonase/DNAse I (Millipore 71205-3) 40% Polyethylene Glycol 8000 (PEG) + ...