We narrowed to 700 results for: BASE;

...whether you will create an N- or C-terminal fusion based on your experimental needs. Equally important, before...

... it, could allow idea generation and extension, based on existing, published data. The importance of data...

... make. The precise mass you measure out will be based on the number of plates you’d like to pour. For ...

...physical titer of samples (viral genomes (vg)/mL) based on the standard curve and the sample dilutions. ...efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal: all samples will have some small amount...

...monoclonal lines generated using this protocol will vary based on several factors, including the growth properties...

... 2: Buffer exchange Choose Option 1 or Option 2 based on the concentration of the pooled sample above....

... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...

...reporters have limited use in vivo because they are based on light which is very easily scattered by tissue...

... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope...

...discipline are welcome. Up to this point most videos are based on research that has been published, but many discuss...

...representing ~85% of the postdoctoral workforce. Based on our communications with and research on these...

...should first develop primers to the approximately 500 base pair sequence surrounding your gRNA. Once amplified...

...could reveal something fascinating. Behavior is based primarily on careful observation of what an animal...

...The dilution series outlined in this protocol are based on an AAV titer range of 5E+12–5E+13 GC/mL, where...

... but care must be taken to perform adequate NGS based QC to ensure no change in representation compared...

... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world...

... smaller, with the full sequence being around 60 base pairs. This is much shorter than miRNA plasmids,...

...fail to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies...

...The dilution series outlined in this protocol is based on an LV titer range of 1E+05–1E+09 TU/mL, where...