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Addgene
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  6. Bioinformatics at Addgene

    Type
    Blog Post
    ...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

  12. Pouring LB Agar Plates

    Type
    Protocol
    ... make. The precise mass you measure out will be based on the number of plates you’d like to pour. For ...

  13. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...physical titer of samples (viral genomes (vg)/mL) based on the standard curve and the sample dilutions. ...efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal: all samples will have some small amount...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...
Showing: 601 - 620 of 705 results