We narrowed to 710 results for: BASE;

1. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   ...development called Scrum. The Scrum framework is based on an iterative and collaborative approach to development...

2. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   ...particularly important if your amplicon is longer than 150 base pairs. While we use ddPCR for AAV titration, there...

3. Hot Plasmids: Winter 2025

   Type

   Blog Post

   ...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...

4. Lab to Office Culture Shock

   Type

   Blog Post

   ...desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone...

5. Plasmids 101: Codon usage bias

   Type

   Blog Post

   ...amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. ... at all in E. coli (you can find a variety of databases showing various organisms’ codon preferences online...

6. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   ...together a really great solution that covered all the bases. You may have also used your writing skills to convey...

7. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   ...you do? What follows are just a few suggestions based on what I have found to be especially beneficial...

8. Healthcare Consulting: A Door to the Business of Life Sciences

   Type

   Blog Post

   ...resources towards the development of a new therapy based on its market need and anticipated commercial success...

9. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   ... 1a). Other strategies that have been developed based off of this concept include: Self-labeling tags...

10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    ...Ohashi, T., et al., An experimental study of GFP-based FRET, with application to intrinsically unstructured...

11. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    ...whether you will create an N- or C-terminal fusion based on your experimental needs. Equally important, before...

12. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    ... it, could allow idea generation and extension, based on existing, published data. The importance of data...

13. Using AAV for Neuronal Tracing

    Type

    Blog Post

    ... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

14. Pouring LB Agar Plates

    Type

    Protocol

    ... make. The precise mass you measure out will be based on the number of plates you’d like to pour. For ...

15. AAV Titration by qPCR Using SYBR Green Technology

    Type

    Protocol

    ...physical titer of samples (viral genomes (vg)/mL) based on the standard curve and the sample dilutions. ...efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal: all samples will have some small amount...

16. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type

    Protocol

    ...monoclonal lines generated using this protocol will vary based on several factors, including the growth properties...

17. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ... 2: Buffer exchange Choose Option 1 or Option 2 based on the concentration of the pooled sample above....

18. Allen Institute for Brain Science AAV Enhancer Collection

    Type

    Collection

    ...in mice. Enhancers were selected from the genome based on their differential chromatin accessibility across...

19. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    ...reporters have limited use in vivo because they are based on light which is very easily scattered by tissue...

20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...