We narrowed to 654 results for: CHI;

1. New Tools Enable CRISPRa for Neuroscience Applications

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   Blog Post

   ...protein. To demonstrate the specificity that can be achieved with CRISPRa, the Day lab singled out two Bdnf...

2. Designing Your Chalk Talk for the Academic Job Interview

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   Blog Post

   ...in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims should have short titles,...

3. Lab to Office Culture Shock

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   Blog Post

   ...tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of camaraderie again...

4. Plasmids 101: Gateway Cloning

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   ... cloning plasmids here! References 1. Chee JY, Chin CF (2015) Gateway Cloning Technology: Advantages...

5. Hot Plasmids: Fall 2024

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   ... abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies...

6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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   Blog Post

   ...disrupt a catalytic residue. Good news! You can achieve your precise edit without even making a break.  ...

7. Bioinformatics at Addgene

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   Blog Post

   ... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

8. New CRISPR Tools: Cas7-11 and PASTE

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   Blog Post

   ...system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It was also ...

9. Antibodies 101: Immunoprecipitation

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   Blog Post

   ...studying epigenetics routinely use the chromatin IP (ChIP) technique to identify DNA binding proteins involved...

10. How to Be an Excellent Trainee

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    Blog Post

    ...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...

11. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    ...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...

12. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post

    ...vision of the final vector structure you want to achieve before starting your experiments. Don’t dive directly...

13. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

    ...benefit when recruiting patients, specifically children and patients with skin disorders for research ...

14. Career Insights: Technical Support Specialist

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    Blog Post

    ...rewarding parts of the job. Solutions may also be achieved by simply replacing the product in question. This...

15. Getting the Most from Your Lentiviral Transduction

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    ...mycoplasma can be extremely hard to detect with levels reaching 108 cells per ml before the media becomes cloudy...

16. Important Considerations in Optogenetics Behavioral Experiments

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    ...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...

17. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post

    ...expression, and viability. Gates are the parameters the machine uses to differentiate between variations in those...

18. CRISPR 101: Validating Your Genome Edit

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    Blog Post

    ... or blog on optimizing this step, or consider enriching for transfected cells by flow sorting or drug ...

19. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post

    ...within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact...

20. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post

    ... stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella Lab devised...