We narrowed to 650 results for: CHI;
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TypeBlog Post...supporting novice to expert-level researchers in achieving consistently successful CRISPR experiments. Jessica...
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Plasmids 101: Gateway Cloning
TypeBlog Post... cloning plasmids here! References 1. Chee JY, Chin CF (2015) Gateway Cloning Technology: Advantages... -
Bioinformatics at Addgene
TypeBlog Post... programs to a uniform language. The modern architecture of Nextflow included built-in support for containers... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It was also ... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...studying epigenetics routinely use the chromatin IP (ChIP) technique to identify DNA binding proteins involved... -
How to Be an Excellent Trainee
TypeBlog Post...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the... -
Hot Plasmids: Fall 2024
TypeBlog Post... abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...disrupt a catalytic residue. Good news! You can achieve your precise edit without even making a break. ... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...benefit when recruiting patients, specifically children and patients with skin disorders for research ... -
Career Insights: Technical Support Specialist
TypeBlog Post...rewarding parts of the job. Solutions may also be achieved by simply replacing the product in question. This... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...mycoplasma can be extremely hard to detect with levels reaching 108 cells per ml before the media becomes cloudy... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...expression, and viability. Gates are the parameters the machine uses to differentiate between variations in those... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post... stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella Lab devised... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...your particular setup. We recommend starting by searching the literature to see what is already known about... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post... or blog on optimizing this step, or consider enriching for transfected cells by flow sorting or drug ... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...usually see a director sitting in their chair not watching their actors live on set, but peering at a 7-inch... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific protein folding...