CRISPR-mediated Plant Base Editors
Type
Blog Post
...deaminate cytosine placed in 2-6 bp and 4-8 bp, respectively, of the protospacer.
And if the target cytosine...
We narrowed to 651 results for: ESP
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Plasmids 101: Plasmid Incompatibility
TypeBlog Post...References del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication and Control... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...chunks, we have the flexibility to adapt and easily respond to changing needs of the teams at Addgene. Another... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...Cells Induces Gamma Rhythm and Controls Sensory Responses.” Nature 459(7247): 663–67. PubMed PMID: 19396156... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...titer virus is used, it is not always feasible, especially when the lentiviral stock needs to be titrated... -
Plasmids 101: Control Plasmids
TypeBlog Post...may be, proper controls are a basic tenet of responsible scientific inquiry and investigation. References... -
Tips for Using FRET in Your Experiments
TypeBlog Post...stoichiometry of donor:acceptor is difficult to address especially when you investigate unknown molecular complexes... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog Post...produced synonymous changes, meaning the new codon corresponded to the same amino acid. Thus, bystander editing... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their emission spectra. These... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...a fluorophore, not every marker will have a corresponding fluorophore-conjugated antibody commercially... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene products were... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...activate the Rho GTPases Cdc42, Rac1, and RhoA, respectively) using the iLID system (Mahlandt et al., 2023... -
Transferable Skills Guide: Time Management
TypeBlog Post...used to do mammalian cell culture, I would often respond to friends asking me to dinner saying something... -
Five Popular Model Organisms
TypeBlog Post...models of human AML accurately predict chemotherapy response." Genes & development 23.7 (2009): 877-889. PubMed... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...downstream of the promoter in Brainbow 1.0 and 1.1, respectively, cells will fluoresce either red or orange until... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...only saw incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...possible are present in most commercial strains, especially mutations that make major improvements such as... -
Addgene's eBook Collection
TypeGuide...CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español January 2024... First Edition, trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close...