...possible are present in most commercial strains, especially mutations that make major improvements such as...

...correlate with catalytic activity at those sites, especially when DNA mismatches are involved. Two other approaches...

...transcriptional activation more than dCas9-VP64, especially when targeting distal enhancers. As the effects...

...Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ...

..., Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator...

...for integration at regions of open chromatin, especially at promoter or exonic regions. If the cell’s ...

... very strong beliefs in traditional medicine, especially when I knew that there was a cheap and easy treatment...

...Overview Bacteria have an interesting adaptive immune response that detects and eliminates foreign DNA, which...fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the HNH/RuvC...sequence The sequence within the gRNA that is responsible for Cas9 binding, it does not include the 20 ...

... HaloTag reacts faster for some dyes, others, especially some nonfluorescent ligands, react more efficiently...

...fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase can still ...

...present-day frogs via genetic engineering. The corresponding movie came out in 1993 and sequels of Jurassic...

... precursors of rice and Arabidopsis species, respectively. Short hairpin RNA (shRNA) is conceptually similar...

... problem. It is estimated that mycoplasma is responsible for up to 60% of the cell culture contamination...

... likely than viral vectors to induce an immune response and are also easier and cheaper to generate. Gene...

...family-wise error and the false discovery rate, respectively. Many other tests exist to identify significant...

...expression of the int and xis genes which are responsible for excision of mini-λ from the host’s chromosome...

...attention to protein modifications which can be especially difficult in alternative expression systems such...

...any neuroscience project or topic of interest. Despite its power and versatility, the technique is remarkably...

..., and guanine (abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix... selective pressure to retain the plasmid DNA, despite the added replication burden, as bacteria without...

...strand that does not base pair with the guide RNA. Despite being called the "non-target strand" in CRISPR ...