We narrowed to 637 results for: ESP

1. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ...a fluorophore, not every marker will have a corresponding fluorophore-conjugated antibody commercially...

2. Transferable Skills Guide: Time Management

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   Blog Post

   ...used to do mammalian cell culture, I would often respond to friends asking me to dinner saying something...

3. Quick Guide to Near-Infrared Fluorescent Proteins

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   Blog Post

   ...miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their emission spectra. These...

4. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   Blog Post

   ...physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene products were...

5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   Blog Post

   ...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic...

6. Five Popular Model Organisms

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   Blog Post

   ...models of human AML accurately predict chemotherapy response." Genes & development 23.7 (2009): 877-889. PubMed...

7. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   Blog Post

   ...downstream of the promoter in Brainbow 1.0 and 1.1, respectively, cells will fluoresce either red or orange until...

8. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   Blog Post

   ...only saw incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our...

9. Thoughts on the Future of Research 2015 Boston Symposium

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   Blog Post

   ...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...

10. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    ...possible are present in most commercial strains, especially mutations that make major improvements such as...

11. Addgene's eBook Collection

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    Guide

    ...CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español January 2024... First Edition, trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close...

12. CRISPR 101: Off-Target Effects

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    Blog Post

    ...correlate with catalytic activity at those sites, especially when DNA mismatches are involved. Two other approaches...

13. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ..., Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator...

14. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post

    ...Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ...

15. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...transcriptional activation more than dCas9-VP64, especially when targeting distal enhancers. As the effects...

16. PiggyBac-ing Through the Genome Editing Field

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    Blog Post

    ...for integration at regions of open chromatin, especially at promoter or exonic regions. If the cell’s ...

17. Finding Your Perfect Job After University

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    Blog Post

    ... very strong beliefs in traditional medicine, especially when I knew that there was a cheap and easy treatment...

18. Deep Dive: Statistical Tests (Comparisons)

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    Blog Post

    ...family-wise error and the false discovery rate, respectively. Many other tests exist to identify significant...

19. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase can still ...

20. Plasmids 101: Using Transposons in the Lab

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    Blog Post

    ... likely than viral vectors to induce an immune response and are also easier and cheaper to generate. Gene...