We narrowed to 672 results for: FIE

1. CRISPR Antimicrobials

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   Blog Post

   ...fashion with the introduction of antibiotics, but the field is currently undergoing a renaissance. Yosef et ...

2. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   Blog Post

   ...tube’s appropriate location. The barcode also identifies the contents of each individual tube.  If a plate...

3. The Importance of a Fun Workplace: Company Culture at Addgene

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   Blog Post

   ...that rut! We’ve done everything from kayaking, to field day, to relay triathlons, to 5Ks. Many of these ...

4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ... active. Further, GFP can label transgenically modified ES cells, which can then be used for implantation...

5. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   ...be at every school performance, chaperone every field trip or cheer at every soccer game. Tip: take turns...

6. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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   Blog Post

   ... Panda, D., Baig, M. J., & Molla, K. (2025). A unified protocol for genome editing in monocot and dicot...

7. Hot Plasmids: Fall 2024

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   Blog Post

   ... This construct can be easily expressed and purified from E. coli, retains important functional properties...

8. Hot Plasmids Spring 2024

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   Blog Post

   ...Cre-expressing vector resulted in a sparsely-labeled field of view that was more desirable than having many...

9. Antibodies 101: Reading a Flow Plot

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   Blog Post

   ... population of interest has been successfully identified and isolated.  Following a gating strategy Figure...

10. Plasmids 101: Codon usage bias

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    Blog Post

    ... (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 for example...

11. Transferable Skills Guide: External Collaborations

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    Blog Post

    ...efforts beyond your organization and/or scientific field. Reaching out to people who have little in common...

12. Transferable Skills Guide: Creativity

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    Blog Post

    ...In Felicia Day’s book Embrace your Weird, Day identifies nine enemies that stand in the way of creative...

13. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...translatable to the endogenous system and will need to be verified using alternative methods.  Finally, IP can also...

14. Viral Vectors 101: Pseudotyping

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    Blog Post

    ...F-L (2002) Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability...

15. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post

    ...by CRISPR/Cas9 in wild-type human iPSCs. Image modified from Le et al., 2019. Gene editing via CRISPR...

16. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post

    ...seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In general split AAVs...

17. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post

    ...Christine Nusslein-Volhard and Eric Wiechaus first identified the hedgehog gene in Drosophila. They observed...

18. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post

    ...such as these has brought a lot of promise to a field that has historically been full of bad news and ...

19. 5 Reasons to Use Reddit for Science Communication

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    Blog Post

    ...with people who work in many different scientific fields. They also host AMA forums where users can ask ...

20. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post

    ...encode N-glycosylation consensus sequences are modified with the addition of N-glycans [9]. Both of these...