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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: HA

Showing: 601 - 620 of 856 results

  1. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...newest viral vector service, AAV Packaged on Request  has seen a fantastic response from the research community...

  3. Summer Fun at Addgene!

    Type
    Blog Post
    ... Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be open to dancing...

  4. Our New Antibody Mascot is....

    Type
    Blog Post
    ...fact, they’ve been following a set of clues that has led them to their biggest discovery yet: Addgene ...

  7. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...as they pass our standard QC process and the MTA has been approved. The spreadsheet option is recommended...cloned into the vector backbone). If your plasmid has more than one insert, click "Save and Add Another...primers can be used to verify it. If your plasmid has multiple inserts, enter details for each insert individually...

  8. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...technology and rapid improvements in the field, it has recently become possible to introduce DSBs and concomitant...efficiency across different stem cell types. This has facilitated easy generation of clonal knock-in lines...or try out an alternative tag (3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely...

  11. Editor's Choice, October 2016

    Type
    Blog Post
    ... of excitement at Addgene. Our new viral service has begun to pick up steam (we already have over 70 orders...

  15. Typing CRISPR Systems

    Type
    Blog Post
    ...these adaptive bacterial and archaeal immune systems has led to extreme sequence divergence that requires ...landmark publication in 2013. Since that point, it has become the most engineered Cas enzyme, with adaptations... CRISPR type that exclusively targets RNA. Cas13 has become a natural successor to traditional RNA interference...

  16. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...bridge helix. Created with BioRender.com.   Cas9 has two major lobes, called NUC (for nuclease) and REC...gray). Target DNA strand, light gray.   Once it has found a PAM site, Cas9 needs to check for complementarity...orange) and HNH (red) domains, while the REC lobe has been hidden for visibility. A RuvC loop caps the ...

  17. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...mycoplasma contamination. Mycoplasma contamination has been shown to arise from a variety of sources such...line for mycoplasma and discard it if the culture has become overly confluent or too old. Ensuring that...

  18. An Introduction to Adenovirus

    Type
    Blog Post
    ... virulence (Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation...the infection to sometimes result in cell death, has led to interest in using adenoviral vectors for vaccine...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...eBook, How to Get a Faculty Position. This edition has substantial expansions on the chalk talks and Zoom...I also incorporated example documents. This part has generated a lot of positive feedback. Many of these...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made...
Showing: 601 - 620 of 856 results