We narrowed to 771 results for: LIS

1. Lab Automation at Addgene

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   Blog Post

   ... of pushing them in one by one. It seems very simplistic and it isn’t electronic but it is one of the ...

2. A Look at Addgene's QC Process

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   Blog Post

   ...Any tags, selectable markers, and fusion proteins listed for each plasmid  If the plasmids pass all inspection...

3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   Blog Post

   ...Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 ...

4. Hot Plasmids February 2024

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   Blog Post

   ...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...

5. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ...Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017...

6. Tips for Technical Support Calls

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   Blog Post

   ...take to get an answer, consider asking to avoid unrealistic expectations. And finally, provide feedback...

7. Gaining Leadership Skills Volunteering at a Professional Organization

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   Blog Post

   ...were held during the weekend. I attended multiple CLIs and benefited greatly by improving my skills and...

8. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...makerspaces around the world. Hackerspaces.org maintains a list of active spaces near you.   Learning about C. ...

9. Hot Plasmids and Viral Preps - May 2021

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   ...synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence, and suspension...

10. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

    ...protein, we have used Western blots, dot blots, ELISAs, and enzyme assays to select both over- and under-expressing...

11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post

    ... stocks that are available to everyone. Flybase lists what stocks are available at Bloomington, but you...

12. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ..., M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023). Transcriptional...

13. Common Injection Routes in Mice

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    Blog Post

    ... for you — and the mice. While not an exhaustive list, these can include what’s being injected, the type...

14. Production of Virus in Insect Versus Mammalian Cells

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    ...Tompkins, G. J., & McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera ...

15. Magnetic Control of Proteins: More than a Dream

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    Blog Post

    ...range of topics. He embraces open science, self-publishing directly on his website (and often posting updates...

16. Transferable Skills Guide: Managing a Team

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    Blog Post

    ...  Equally as important is to be able to actively listen - this can be hard - but it is the best way to ...

17. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

18. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ...gene of interest will direct the investigator to a list of all available Addgene plasmids he or she can ...

19. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ...

20. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    ...dimeric FP could ruin your day (and experiment) are listed below. Solutions to avoid these all too common ...