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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 851 results for: Met

Showing: 601 - 620 of 851 results

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...degron tags can offer over other protein control methods and compare the commonly available types of degron...protein post-translationally, unlike many other methods. Secondly, these tags can be used to manipulate...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...from contaminants using a variety of purification methods. Deciding which expression system to use depends...transiently or through stable cell lines. Both methods produce high protein yields if transfection is ...

  5. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...down the question you were trying to answer, the method you used, the main result, and the fundamental ...you’ve already set yourself up for success. But sometimes, attendees can get sucked in to thirty-minute-...

  6. PhD Applications After COVID

    Type
    Blog Post
    ...speaking, the PhD application process tends to go something like this:   However, as simply as it seems laid...program. An email to a potential PI should go something like this: Introduce yourself, brief educational...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...these electrical events! Voltage indicators are voltmeters for cells! In this blog we will review how these... described systems. Several adaptations of this method have been to tag, cage, uncage, and trap the chemical...

  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...relationship is non-linear and you should use a different method of analysis. Different fields and assays have varying...

  10. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...it at the time, but gene therapy had fallen - plummeted really -  from grace 2 years prior with the death...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

  11. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...developed into lentiviral vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen...viruses and transduction by vectors (which we sometimes still call “infection” as a shorthand, although...

  12. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...many facts but the training of the mind to think something that cannot be learned from textbooks” (Brian,...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...

  13. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...step (Moreno-Mateos, et al., Nat Com). Another method used to prime a Cas break for HR is to fuse an ...jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed repair choice in response...

  14. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...receive include the sense that people learned something new? Did the meeting begin and end on time? Did...

  16. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct... populations in drug screens, to visualize micrometastases in nude mice in cancer studies, act as a reporter...

  19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...essential for this task. However, most lesioning methods destroy areas larger than intended and may unintentionally...with far fewer side effects compared to lesioning methods -  they may be the key to further unraveling the...

  20. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...improves expression levels. Journal of Neuroscience Methods 124:75–81 . https://doi.org/10.1016/s0165-0270(...base-pairing slows in vivo translation elongation in metazoans. RNA 17:2063–2073 . https://doi.org/10.1261/rna...
Showing: 601 - 620 of 851 results