We narrowed to 840 results for: Met

...step (Moreno-Mateos, et al., Nat Com). Another method used to prime a Cas break for HR is to fuse an ...jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed repair choice in response...

...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...receive include the sense that people learned something new? Did the meeting begin and end on time? Did...

...barcoded tube (see next section) and every time something is removed from this tube, the barcode on the ...digit barcode and then assigned to a location in a metal freezer rack that contains five levels that can ...

...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct... populations in drug screens, to visualize micrometastases in nude mice in cancer studies, act as a reporter...

...Biospace holds career events all over the country; sometimes several per month, giving you a plethora of opportunities... Post your CV and make it visible, which will sometimes result in a recruiter giving you a call. Many ...

...companies have dedicated technical support teams, sometimes comprised of several layers of support: A front-line...listen to the many individual stories and get (sometimes refreshing) insights into how other scientists...

...improves expression levels. Journal of Neuroscience Methods 124:75–81 . https://doi.org/10.1016/s0165-0270(...base-pairing slows in vivo translation elongation in metazoans. RNA 17:2063–2073 . https://doi.org/10.1261/rna...

...of base-edited cell populations. To develop this method the lab was inspired by work that used an integrated...fluorescence that can be visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine...

...essential for this task. However, most lesioning methods destroy areas larger than intended and may unintentionally...with far fewer side effects compared to lesioning methods -  they may be the key to further unraveling the...

...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many... editing in rice and uses base editors and HDR methods for rice crop improvement in the laboratory of ...

..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels... of proximity labeling in living cells. Nature Methods, 20(6), 908–917. doi: 10.1038/s41592-023-01880-...

...vectors. While viral delivery is a highly effective method for gene editing and allows targeting of specific...annealed oligos into the digested vector using your method of choice. Step 5: Colony Screening Use Primer...

...enzymes (BsaI, BbsI/BpiI, BsmBI), which recognize asymmetric DNA sequences and cleave at a short distance ...stocks beforehand. You can also use high-throughput methods to streamline your plasmid extractions. Use this...

..., or pretty much anyone applying the scientific method to a question. Videos from any discipline are welcome...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...

...goal posts” as a part of a case, consulting can sometimes be challenging. The fast paced nature of projects...of great output in a short amount of time can sometimes become cauldrons on the verge of boiling. On other...

...Tends to resist promoter down regulation due to methylation or deacetylation. Ubc General expression mRNA... General expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite...

...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...Obstacles for CRISPR HIV-1 therapy Both of these methods represent exciting advances in preclinical HIV-...

...equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all the major equipment...their success. Therefore, assume positive intent. Sometimes, “no we don’t have the funds,” really means they...

... body works, generating new therapeutics, and sometimes formulating new biological concepts. However, ... technique simply by following the abbreviated methods section found within a published manuscript. Luckily...

...technology, and the user may need to optimize numerous parameters to generate the desired edit. The wrench: Gene...universal scoring system for selecting a gRNA, as the method of producing the guide (synthetic, in vitro transcription...