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We narrowed to 876 results for: NSI

Showing: 601 - 620 of 876 results

  2. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...abundance of your targets isn’t too different, consider antibody affinity: stronger binding antibodies...membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won’t evenly re-absorb...

  3. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...the ‘quality’ of the gRNA sequence, including propensity for off-targets, secondary structures, etc., ...more pan-genome PAM flexibility, you’ll want to consider one of several Cas9s that recognize NG PAMs. The...

  5. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...lack all viral genes, including those that are responsible for integration into host chromosomes, further...to other gene delivery tools that are under consideration, recent advancements in various AAV technologies...

  6. 6 Tips for Grant Writing

    Type
    Blog Post
    ...in different fields that can provide different insight into an area - not just working within the established...collaborators can also help you get some critical insight into what you're doing wrong. All this helps to...

  10. AAV ddPCR Titration

    Type
    Protocol
    ... 10% bleach. Safety Warnings AAV is generally considered biosafety level 1 but may require BSL-2 handling...45° angle. Count to 20 while slowly and gently dispensing the droplets down the side of the tube. Place... 95 10 2 1 Denaturation 95 0.5 2 50 Annealing/Extension 60 1 2 50 Signal Stabilization 98 10 2 1 Hold ... should be close to zero (B08). At Addgene, we consider runs with an NTC > 10 to be invalid. To reduce...

  11. Of Myc and Men

    Type
    Blog Post
    ...epitope, a phenomenon known as sequence context sensitivity (Schüchner, et al. 2020). In their study, some... newer Myc-tag antibodies appeared to be less sensitive to sequence context.    So, do Schüchner, et al...

  12. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ..., cultures treated with a folA gRNA were more sensitive to trimethoprim when compared to control gRNA ...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...

  13. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...primarily found in the culture media, you should consider performing PEG precipitation. Instead of sonicating...solutions are carefully layered and then the viral suspension generated during AAV production is overlaid. ...

  14. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... increase efficiency of these systems, though consistent expression can still be a challenge. A multitasking...powerful than it is. The nuclease domain of Cas3 is considered weak, especially compared to the power of Cas9...

  15. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...normally lies around 7.35-7.4 so mammalian cells are sensitive to pH. Anything between orange and light orange...Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent organizing...

  16. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...which way your population needs to be shifted, consider your perpendicular lines. In this case, the population...applying very large compensation values (100+), consider what two markers your fluorescent markers are ...

  17. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... 3-D path of the protein’s backbone in three-dimensional space, but their flexible and jiggly nature suggested...structure into experimentally determined electron density, have been known to say something silly like “See...

  18. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...nuclease-null—in order to effect different perturbations. Consider, for instance, the challenge of attempting to ...alterations can be exploited to quickly and inexpensively generate a potent Cas9-based transcriptional...

  19. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...can survive positive selection, but it will be sensitive to negative selection. Remove the positive selection...creating knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures...
Showing: 601 - 620 of 876 results