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We narrowed to 882 results for: NSI

Showing: 601 - 620 of 882 results

  5. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...Special breeding cases Whether your mouse colony consists of genetically engineered mice with a single mutation...strains that have been crossed, there are many considerations for effectively breeding your mice. Because...

  6. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...scientists to confidently choose reliable and consistent antibodies for specific markers. The Screening...production methods    NeuroMab and others have an extensive library of monoclonal antibodies; Addgene collaborates...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...lack all viral genes, including those that are responsible for integration into host chromosomes, further...to other gene delivery tools that are under consideration, recent advancements in various AAV technologies...

  10. AAV Production in HEK293 Cells

    Type
    Protocol
    ... through a 0.22 μm membrane. Store at 4 °C. Considerations Before You Start The health of the HEK293T ...vigorously multiple times to obtain a single cell suspension (no clumps of cells). Pool cells from 2 x T-175...forth or shake vigorously to obtain a single cell suspension (no clumps). Count cells using a hemocytometer...above. Add 50 units of benzonase per mL of viral suspension (you should have ~18 mL from steps 12 & 13). ...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... increase efficiency of these systems, though consistent expression can still be a challenge. A multitasking...powerful than it is. The nuclease domain of Cas3 is considered weak, especially compared to the power of Cas9...

  13. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...which way your population needs to be shifted, consider your perpendicular lines. In this case, the population...applying very large compensation values (100+), consider what two markers your fluorescent markers are ...

  14. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... 3-D path of the protein’s backbone in three-dimensional space, but their flexible and jiggly nature suggested...structure into experimentally determined electron density, have been known to say something silly like “See...

  15. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...nuclease-null—in order to effect different perturbations. Consider, for instance, the challenge of attempting to ...alterations can be exploited to quickly and inexpensively generate a potent Cas9-based transcriptional...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...can survive positive selection, but it will be sensitive to negative selection. Remove the positive selection...creating knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures...

  17. Of Myc and Men

    Type
    Blog Post
    ...epitope, a phenomenon known as sequence context sensitivity (Schüchner, et al. 2020). In their study, some... newer Myc-tag antibodies appeared to be less sensitive to sequence context.    So, do Schüchner, et al...

  18. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...primarily found in the culture media, you should consider performing PEG precipitation. Instead of sonicating...solutions are carefully layered and then the viral suspension generated during AAV production is overlaid. ...

  19. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...normally lies around 7.35-7.4 so mammalian cells are sensitive to pH. Anything between orange and light orange...Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent organizing...

  20. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ..., cultures treated with a folA gRNA were more sensitive to trimethoprim when compared to control gRNA ...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...
Showing: 601 - 620 of 882 results