We narrowed to 653 results for: PPL

1. Save Time with Transient Plant Leaf Transformations

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   ...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration...

2. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ...

3. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...noise, of dsDNA breaks caused by CRISPR. See supplemental figure 6 for more details. Safe-targeting guides...

4. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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   ...when discussing raising children, it can also be applied to research. Sharing knowledge and tools is the...

5. CRISPR-mediated Plant Base Editors

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   ...question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016...

6. Mesothelioma - Causes, Symptoms, and Treatment

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   ...pemetrexed and cisplatin –because it cuts off the blood supply to fast-growing cancer tumors. In fact, the use...

7. Using Video to Share Your Science: We Share Science

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   ...private sector researchers, or pretty much anyone applying the scientific method to a question. Videos from...

8. Changing Labor Laws Bring Increased Postdoc Wages

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   ...institutions, or institutions they're thinking about applying to, are doing - for example, your benefits may...

9. The Challenges of Cell Culture

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   ...including GSK, AstraZeneca-MedImmune, and Infors & Applikon. Due to his high level of experience he now travels...

10. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ... of a mystical "Force," this quote is equally applicable to many microscopy experiments with fluorescent...

11. Plasmids 101: E. coli Strains for Protein Expression

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    ...cell-density they support; however, minimal media supplemented with M9 salts may be preferable if the protein...

12. Tips for Using FRET in Your Experiments

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    ...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

13. Data Freedom: The Expansion of Data Sharing in Research Publications

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    ...distinct (perhaps similarly impoverished lab) might apply different or novel tools and assumptions to reveal...

14. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...and may need to be optimized for each unique application (Trapani et al., 2015).  Expression of smaller...

15. CRISPR 101: Validating Your Genome Edit

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    ...sequencing confirmation. All knock-ins A method applicable to knock-ins both large and small is a software...

16. The Fluorescent Vegetables in Aptamer Soup

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    ...aptamer soup by exploring the development and applications of fluorescent RNA aptamers in Addgene’s collection...

17. Fluorescent Proteins 101: When GFP lets you down

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    ... proteins.Therefore, GFP is not suitable for applications that require anaerobic conditions. In experiments...

18. Antibodies 101: Introduction to Gating in Flow Cytometry

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    ...voltage describes an electric potential that can be applied to the photomultiplier inside the cytometer to ...

19. An Integrin Antibody Toolkit from IPI

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    ... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate...

20. 10 Steps to a Perfect Science Talk

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    ...non-academic settings. Many of these guidelines will apply to any type of science presentation, but, for more...