We narrowed to 651 results for: PPL

1. CRISPR 101: Any Base Transversion Editors

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   ...researchers can now select the best tool for their application from among many options.  Conclusion With these...

2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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   ...Motif (PAM), the sequence NGG.  If these conditions apply to your experimental target, SpCas9 and its enhanced...

3. Viral Vectors 101: Pseudotyping

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   ...Wickersham et al., 2007). This system requires first supplying rabies glycoprotein along with the TVA receptor...

4. CRISPR-mediated Plant Base Editors

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   ...question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016...

5. Mesothelioma - Causes, Symptoms, and Treatment

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   ...pemetrexed and cisplatin –because it cuts off the blood supply to fast-growing cancer tumors. In fact, the use...

6. Using Video to Share Your Science: We Share Science

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   ...private sector researchers, or pretty much anyone applying the scientific method to a question. Videos from...

7. Changing Labor Laws Bring Increased Postdoc Wages

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   ...institutions, or institutions they're thinking about applying to, are doing - for example, your benefits may...

8. The Challenges of Cell Culture

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   ...including GSK, AstraZeneca-MedImmune, and Infors & Applikon. Due to his high level of experience he now travels...

9. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   ... of a mystical "Force," this quote is equally applicable to many microscopy experiments with fluorescent...

10. Plasmids 101: E. coli Strains for Protein Expression

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    ...cell-density they support; however, minimal media supplemented with M9 salts may be preferable if the protein...

11. Tips for Using FRET in Your Experiments

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    ...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

12. Data Freedom: The Expansion of Data Sharing in Research Publications

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    ...distinct (perhaps similarly impoverished lab) might apply different or novel tools and assumptions to reveal...

13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...and may need to be optimized for each unique application (Trapani et al., 2015).  Expression of smaller...

14. Fluorescent Proteins 101: When GFP lets you down

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    ... proteins.Therefore, GFP is not suitable for applications that require anaerobic conditions. In experiments...

15. The Fluorescent Vegetables in Aptamer Soup

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    ...aptamer soup by exploring the development and applications of fluorescent RNA aptamers in Addgene’s collection...

16. Antibodies 101: Introduction to Gating in Flow Cytometry

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    ...voltage describes an electric potential that can be applied to the photomultiplier inside the cytometer to ...

17. An Integrin Antibody Toolkit from IPI

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    ... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate...

18. CRISPR 101: Validating Your Genome Edit

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    ...sequencing confirmation. All knock-ins A method applicable to knock-ins both large and small is a software...

19. 10 Steps to a Perfect Science Talk

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    ...non-academic settings. Many of these guidelines will apply to any type of science presentation, but, for more...

20. Site Directed Mutagenesis by PCR

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    ...of CRISPR; site-directed mutagenesis generally applies to plasmids, but may also facilitate genome editing...