We narrowed to 741 results for: REV

1. Antibodies 101: Selecting the Right Antibody

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   Blog Post

   ...can narrow them down in a variety of ways. First, review the citations. An antibody that has been cited ...

2. Antibodies 101: Secondary Antibodies

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   ...the antibody binds to a protein, and then voila! Wherever your protein of interest is, a signal will also...

3. Transferable Skills Guide: Problem Solving

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   ...strategy was. If you didn’t quite fix everything or revealed another issue in the process, you might need to...

4. What's New in CRISPR - November 2021

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   ... PE-Cas9 comprises an active Cas9 fused to the reverse transcriptase (RT), which induces two double-strand...

5. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   ...(PV)-expressing neurons, neurons that the lab previously found had a role in perceiving touch inputs. ...

6. Antibodies 101: Polyclonal Antibodies

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   ...stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their batch...

7. RNA Extraction Without A Kit

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   ...or TE buffer, store them in a -80°C freezer to prevent RNA degradation. They will degrade in a -20°C freezer...

8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...expression and reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems (ex: tamoxifen...

9. The time and cost required to make a plasmid

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   ..., you’ll be able to use or build on work done previously, borrow small aliquots of restriction enzymes...

10. dTAG - You're it!

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    ...the proteasome to destroy targeted proteins. We previously developed small molecule degraders that achieve...

11. RANbodies: Reporter Nanobody Fusions

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    ...To create their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the...

12. Human Germline Editing Using CRISPR

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    ...germline editing is permissible in cases of disease prevention. However, it is important to consider that already-tested...

13. Visualizing Protein Turnover In Situ

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    ...Singer. "Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability....

14. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...their own unique features and quirks. Here, we will review the genome engineering abilities of the lesser-...

15. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    ...for this purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific...

16. Plasmids 101: Golden Gate Cloning

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    ...formation of the desired ligation product is irreversible because this construct does not retain the enzyme...

17. Career Coaching for Scientists: Why and Where Do I Find One?

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    ...suggestions.  Most coaches have websites you can review and most create useful online content in the form...

18. Working with Nuclear Receptors

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    ...regulatory molecules, and has provided clues to their previously uncharacterized cellular roles. In the current...

19. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...expression plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA...

20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection...