We narrowed to 716 results for: cas

1. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   ...Recombinant AAV vector with ITRs that flank the gene cassette, which contains a promoter, the gene of interest...

2. Four Factors that Differentiate the Stem Cell Field

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   ...scientific method cannot address. Listen to Our Podcast Interview with Stem Cell Researcher Darrell Kotton...

3. Management for Scientists: Delegating is Key

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   ...process is completed more efficiently. Listen to our podcast interview with Harvard Medical School researcher...

4. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   ...ones), they can be a good starting point. In many cases it is sufficient to use a spectrum for a closely...

5. Advice for Scientists Starting a Lab

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   ...“off flying his plane to exotic places while occasionally emailing us his worldly advice”, the other was...

6. Why and how to start studying chromatin (with Addgene's help)

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   ...CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics...

7. A Novice's Perspective on Neuroscience - SfN2017

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   Blog Post

   ...be molecular biologists. This was not at all the case. The conference featured people who work in sociology...

8. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   ...Transformation of Drosophila via PhiC31 Integrase-Mediated Cassette Exchange. Genetics. 173, 769–777 (2006). PubMed...

9. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   ... EGFP and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact...

10. Luminescent Imaging with Nano-lanterns

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    Blog Post

    ... endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging ...

11. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    ...extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot like a game of telephone...

12. Plasmids 101: Screens vs. Selections

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    ... Genetic screens in human cells using the CRISPR/Cas9 system. Science, 343(6166): 80-84 (2014). doi: https...

13. AAV Q&A with Tim Miles

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    ...primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org...

14. Sweating the Small Stuff: Details in the Lab

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    ...before, which got rid of the issue entirely. In many cases, the answer isn't to work harder - it's to work ...

15. Antibodies 101: Buffers, Storage, and Conjugates

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    ...sodium azide at all, as it inhibits HRP. In those cases, thimerosal at a concentration of 0.01% w/v can ...

16. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...that share homology in the same reaction. In these cases, the use of another cloning method, such as Golden...

17. Viral Vectors 101: Types of viruses

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    ...large enough for many applications, but in some cases multiple AAVs (co-infection), an alternative virus...

18. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post

    ...representations of the 3D models, which in many cases can be produced quickly with the printer, act here...

19. 7 Tips to Secure a STEAM Internship This Summer

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    Blog Post

    ... personal statement, while you make the ultimate case: why you? 7. Strength in numbers Encourage your ...

20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ...were conducted on how the results were analyzed. A casual reading of the study is not enough to uncover these...